Neutralization of human thyrotropin by antisera to subunits of glycoprotein hormones

B. C. Nisula, P. O. Kohler, J. L. Vaitukaitis, J. M. Hershman, G. T. Ross

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The effects of antisera directed against various human glycoprotein hormone subunits on the biologic activity of human pituitary thyrotropin (hTSH), human chorionic thyrotropin (hCT), bovine pituitary thyrotropin (bTSH1), and long-acting thyroid stimulator (LATS) were studied. Relative thyroid-stimulating potencies were determined using a McKenzie mouse assay. Antiserum to the human chorionic gonadotropin a-subunit (hCGα) completely neutralized hTSH but not hCT, bTSH, or LATS. Antisera to the hCG β-subunit (hCGβ) only partially neutralized hTSH and had no inhibiting effect on the biologic activity of hCT, hTSH, and LATS. In contrast, antisera to the human luteinizing hormone β-subunit had no significant effect on hTSH biologic activity. These results confirm previous reports indicating common antigenic determinants on hCGα subunit and hTSH and suggest similarity of the a subunits of hCG and hTSH. Placental thyroid-stimulating activity was not neutralized by either anti-hCGα or anti-hCGβ sera, indicating that hCT is immunologically distinct from hTSH and hCG, and suggesting that hCT does not have an α-subunit structure immunologically similar to that in the other human glycoprotein hormones.

Original languageEnglish (US)
Pages (from-to)664-669
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume37
Issue number5
DOIs
StatePublished - Nov 1973

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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