Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications

Barrett J. Nehilla, Ketul C. Popat, Tothu (Tania) Vu, Sarwat Chowdhury, Robert F. Standaert, David R. Pepperberg, Tejal A. Desai

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The design of chemically well-defined, machinable surfaces containing neuroactive molecules offers potential for fundamental neuroscience and clinical neural engineering applications. Here we report the assembly and characterization of silicon platforms containing a tethered form of muscimol. Muscimol, an analog of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), is a potent agonist at postsynaptic GABAA and GABA C receptors. Surfaces were assembled using covalent avidin conjugation to silanized silicon followed by high-affinity avidin-biotin binding of a biotinylated derivative of muscimol (muscimol-biotin). Contact angle measurements, ellipsometry, and X-ray photoelectron spectroscopy (XPS) were conducted to characterize the wettability, thickness, and chemical composition of progressively deposited surface layers. The data demonstrate successful incorporation of a neurotransmitter analog as part of a layered, silicon-based structure possessing robust and specific biomolecular composition. These findings represent a step toward the design of platforms for applications involving control and modulation of neural signaling.

Original languageEnglish (US)
Pages (from-to)669-674
Number of pages6
JournalBiotechnology and Bioengineering
Volume87
Issue number5
DOIs
StatePublished - Sep 5 2004
Externally publishedYes

Fingerprint

BioMEMS
Micro-Electrical-Mechanical Systems
Muscimol
Silicon
Neurotransmitter Agents
Avidin
Biotin
Ellipsometry
Angle measurement
Biomedical Engineering
Aminobutyrates
Wettability
Chemical analysis
Photoelectron Spectroscopy
Contact angle
Wetting
X ray photoelectron spectroscopy
Neurosciences
Modulation
gamma-Aminobutyric Acid

Keywords

  • Avidin-biotin
  • BioMEMS
  • Muscimol
  • Neurotransmitter
  • Silicon
  • Surface analysis

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Nehilla, B. J., Popat, K. C., Vu, T. T., Chowdhury, S., Standaert, R. F., Pepperberg, D. R., & Desai, T. A. (2004). Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications. Biotechnology and Bioengineering, 87(5), 669-674. https://doi.org/10.1002/bit.20171

Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications. / Nehilla, Barrett J.; Popat, Ketul C.; Vu, Tothu (Tania); Chowdhury, Sarwat; Standaert, Robert F.; Pepperberg, David R.; Desai, Tejal A.

In: Biotechnology and Bioengineering, Vol. 87, No. 5, 05.09.2004, p. 669-674.

Research output: Contribution to journalArticle

Nehilla, BJ, Popat, KC, Vu, TT, Chowdhury, S, Standaert, RF, Pepperberg, DR & Desai, TA 2004, 'Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications', Biotechnology and Bioengineering, vol. 87, no. 5, pp. 669-674. https://doi.org/10.1002/bit.20171
Nehilla, Barrett J. ; Popat, Ketul C. ; Vu, Tothu (Tania) ; Chowdhury, Sarwat ; Standaert, Robert F. ; Pepperberg, David R. ; Desai, Tejal A. / Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications. In: Biotechnology and Bioengineering. 2004 ; Vol. 87, No. 5. pp. 669-674.
@article{dadef31f44f641ff9b72e1cbf28ba6bd,
title = "Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications",
abstract = "The design of chemically well-defined, machinable surfaces containing neuroactive molecules offers potential for fundamental neuroscience and clinical neural engineering applications. Here we report the assembly and characterization of silicon platforms containing a tethered form of muscimol. Muscimol, an analog of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), is a potent agonist at postsynaptic GABAA and GABA C receptors. Surfaces were assembled using covalent avidin conjugation to silanized silicon followed by high-affinity avidin-biotin binding of a biotinylated derivative of muscimol (muscimol-biotin). Contact angle measurements, ellipsometry, and X-ray photoelectron spectroscopy (XPS) were conducted to characterize the wettability, thickness, and chemical composition of progressively deposited surface layers. The data demonstrate successful incorporation of a neurotransmitter analog as part of a layered, silicon-based structure possessing robust and specific biomolecular composition. These findings represent a step toward the design of platforms for applications involving control and modulation of neural signaling.",
keywords = "Avidin-biotin, BioMEMS, Muscimol, Neurotransmitter, Silicon, Surface analysis",
author = "Nehilla, {Barrett J.} and Popat, {Ketul C.} and Vu, {Tothu (Tania)} and Sarwat Chowdhury and Standaert, {Robert F.} and Pepperberg, {David R.} and Desai, {Tejal A.}",
year = "2004",
month = "9",
day = "5",
doi = "10.1002/bit.20171",
language = "English (US)",
volume = "87",
pages = "669--674",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "5",

}

TY - JOUR

T1 - Neurotransmitter analog tethered to a silicon platform for neuro-BioMEMS applications

AU - Nehilla, Barrett J.

AU - Popat, Ketul C.

AU - Vu, Tothu (Tania)

AU - Chowdhury, Sarwat

AU - Standaert, Robert F.

AU - Pepperberg, David R.

AU - Desai, Tejal A.

PY - 2004/9/5

Y1 - 2004/9/5

N2 - The design of chemically well-defined, machinable surfaces containing neuroactive molecules offers potential for fundamental neuroscience and clinical neural engineering applications. Here we report the assembly and characterization of silicon platforms containing a tethered form of muscimol. Muscimol, an analog of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), is a potent agonist at postsynaptic GABAA and GABA C receptors. Surfaces were assembled using covalent avidin conjugation to silanized silicon followed by high-affinity avidin-biotin binding of a biotinylated derivative of muscimol (muscimol-biotin). Contact angle measurements, ellipsometry, and X-ray photoelectron spectroscopy (XPS) were conducted to characterize the wettability, thickness, and chemical composition of progressively deposited surface layers. The data demonstrate successful incorporation of a neurotransmitter analog as part of a layered, silicon-based structure possessing robust and specific biomolecular composition. These findings represent a step toward the design of platforms for applications involving control and modulation of neural signaling.

AB - The design of chemically well-defined, machinable surfaces containing neuroactive molecules offers potential for fundamental neuroscience and clinical neural engineering applications. Here we report the assembly and characterization of silicon platforms containing a tethered form of muscimol. Muscimol, an analog of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), is a potent agonist at postsynaptic GABAA and GABA C receptors. Surfaces were assembled using covalent avidin conjugation to silanized silicon followed by high-affinity avidin-biotin binding of a biotinylated derivative of muscimol (muscimol-biotin). Contact angle measurements, ellipsometry, and X-ray photoelectron spectroscopy (XPS) were conducted to characterize the wettability, thickness, and chemical composition of progressively deposited surface layers. The data demonstrate successful incorporation of a neurotransmitter analog as part of a layered, silicon-based structure possessing robust and specific biomolecular composition. These findings represent a step toward the design of platforms for applications involving control and modulation of neural signaling.

KW - Avidin-biotin

KW - BioMEMS

KW - Muscimol

KW - Neurotransmitter

KW - Silicon

KW - Surface analysis

UR - http://www.scopus.com/inward/record.url?scp=4644245842&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644245842&partnerID=8YFLogxK

U2 - 10.1002/bit.20171

DO - 10.1002/bit.20171

M3 - Article

VL - 87

SP - 669

EP - 674

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 5

ER -