Neuronal hyperpolarization-activated pacemaker channels drive neuropathic pain

Sandra R. Chaplan, Hong Qing Guo, Doo Hyun Lee, Lin Luo, Changlu Liu, Chester Kuei, Alexander A. Velumian, Matthew Butler, Sean M. Brown, Adrienne E. Dubin

Research output: Contribution to journalArticle

234 Citations (Scopus)

Abstract

Neuropathic pain is a common and often incapacitating clinical problem for which little useful therapy is presently available. Painful peripheral neuropathies can have many etiologies, among which are trauma, viral infections, exposure to radiation or chemotherapy, and metabolic or autoimmune diseases. Sufferers generally experience both pain at rest and exaggerated, painful sensitivity to light touch. Spontaneous firing of injured nerves is believed to play a critical role in the induction and maintenance of neuropathic pain syndromes. Using a well characterized nerve ligation model in the rat, we demonstrate that hyperpolarization- activated, cyclic nucleotide-modulated (HCN) "pacemaker" channels play a previously unrecognized role in both touch-related pain and spontaneous neuronal discharge originating in the damaged dorsal root ganglion. HCN channels, particularly HCN1, are abundantly expressed in rat primary afferent somata. Nerve injury markedly increases pacemaker currents in large-diameter dorsal root ganglion neurons and results in pacemaker-driven spontaneous action potentials in the ligated nerve. Pharmacological blockade of HCN activity using the specific inhibitor ZD7288 reverses abnormal hypersensitivity to light touch and decreases the firing frequency of ectopic discharges originating in Aβ and Aδ fibers by 90 and 40%, respectively, without conduction blockade. These findings suggest novel insights into the molecular basis of pain and the possibility of new, specific, effective pharmacological therapies.

Original languageEnglish (US)
Pages (from-to)1169-1178
Number of pages10
JournalJournal of Neuroscience
Volume23
Issue number4
StatePublished - Feb 15 2003
Externally publishedYes

Fingerprint

Cyclic Nucleotides
Touch
Neuralgia
Spinal Ganglia
Pain
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
Pharmacology
Myelinated Nerve Fibers
Photophobia
Metabolic Diseases
Wounds and Injuries
Carisoprodol
Peripheral Nervous System Diseases
Virus Diseases
Autoimmune Diseases
Action Potentials
Ligation
Hypersensitivity
Maintenance
Neurons

Keywords

  • Dorsal root ganglion
  • Ectopic discharge
  • HCN channel
  • I
  • Neuropathic pain
  • Pacemaker channel
  • Spinal nerve ligation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chaplan, S. R., Guo, H. Q., Lee, D. H., Luo, L., Liu, C., Kuei, C., ... Dubin, A. E. (2003). Neuronal hyperpolarization-activated pacemaker channels drive neuropathic pain. Journal of Neuroscience, 23(4), 1169-1178.

Neuronal hyperpolarization-activated pacemaker channels drive neuropathic pain. / Chaplan, Sandra R.; Guo, Hong Qing; Lee, Doo Hyun; Luo, Lin; Liu, Changlu; Kuei, Chester; Velumian, Alexander A.; Butler, Matthew; Brown, Sean M.; Dubin, Adrienne E.

In: Journal of Neuroscience, Vol. 23, No. 4, 15.02.2003, p. 1169-1178.

Research output: Contribution to journalArticle

Chaplan, SR, Guo, HQ, Lee, DH, Luo, L, Liu, C, Kuei, C, Velumian, AA, Butler, M, Brown, SM & Dubin, AE 2003, 'Neuronal hyperpolarization-activated pacemaker channels drive neuropathic pain', Journal of Neuroscience, vol. 23, no. 4, pp. 1169-1178.
Chaplan SR, Guo HQ, Lee DH, Luo L, Liu C, Kuei C et al. Neuronal hyperpolarization-activated pacemaker channels drive neuropathic pain. Journal of Neuroscience. 2003 Feb 15;23(4):1169-1178.
Chaplan, Sandra R. ; Guo, Hong Qing ; Lee, Doo Hyun ; Luo, Lin ; Liu, Changlu ; Kuei, Chester ; Velumian, Alexander A. ; Butler, Matthew ; Brown, Sean M. ; Dubin, Adrienne E. / Neuronal hyperpolarization-activated pacemaker channels drive neuropathic pain. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 4. pp. 1169-1178.
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