Neuronal elements in the testis of the rhesus monkey: Ontogeny, characterization and relationship to testicular cells

Monica B. Frungieri, Henryk Urbanski, Barbara Höhne-Zell, Artur Mayerhofer

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) were recently identified in the testis of the prepubertal rhesus monkey. In this study, we characterized the neuron-like nature of these cells and examined distribution and frequency of neuronal elements in the testes of monkeys during postnatal development, puberty and adulthood. Using immunohistochemical methods, we detected both nerve fibers and cell bodies, immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP-25), TH and neuropeptide Y (NPY) in perivascular locations, intermingled with interstitial cells and close to the wall of seminiferous tubules. Marked age-related differences in the numbers of these neuronal elements became apparent, when we quantified NF-200-immunoreactive neuronal elements. Thus, intrinsic neuron-like cell bodies were found only in the testes from immature animals (i.e., until about 3 years of age). Conversely, nerve fibers, presumably representing mainly the extrinsic innervation, were observed at all ages although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, another testicular cell type known to contain potent regulatory substances, mast cells, was found to be in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same pattern as nerve fibers. These results confirm that the testicular nervous system of the monkey is composed of two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components show a marked degree of plasticity during development, especially around the time of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides are likely to have multiple sites of actions, and may affect Leydig cells, cells of the tubular wall and vascular cells directly and/or indirectly via intermediation of mast cells.

    Original languageEnglish (US)
    Pages (from-to)43-50
    Number of pages8
    JournalNeuroendocrinology
    Volume71
    Issue number1
    StatePublished - 2000

    Fingerprint

    Macaca mulatta
    Testis
    Nerve Fibers
    Neurons
    Puberty
    Tyrosine 3-Monooxygenase
    Mast Cells
    Catecholamines
    Haplorhini
    Synaptosomal-Associated Protein 25
    Tryptases
    Seminiferous Tubules
    Leydig Cells
    Neuropeptide Y
    Neuropeptides
    Cell Wall
    Nervous System
    Blood Vessels
    Testosterone
    Cell Count

    Keywords

    • Catecholamines
    • Gonads
    • Mast cells
    • Peripheral neuroendocrinology
    • Primates
    • Testis

    ASJC Scopus subject areas

    • Endocrinology
    • Neuroscience(all)

    Cite this

    Neuronal elements in the testis of the rhesus monkey : Ontogeny, characterization and relationship to testicular cells. / Frungieri, Monica B.; Urbanski, Henryk; Höhne-Zell, Barbara; Mayerhofer, Artur.

    In: Neuroendocrinology, Vol. 71, No. 1, 2000, p. 43-50.

    Research output: Contribution to journalArticle

    Frungieri, Monica B. ; Urbanski, Henryk ; Höhne-Zell, Barbara ; Mayerhofer, Artur. / Neuronal elements in the testis of the rhesus monkey : Ontogeny, characterization and relationship to testicular cells. In: Neuroendocrinology. 2000 ; Vol. 71, No. 1. pp. 43-50.
    @article{ff262efd781243afb16a422a35ad60da,
    title = "Neuronal elements in the testis of the rhesus monkey: Ontogeny, characterization and relationship to testicular cells",
    abstract = "Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) were recently identified in the testis of the prepubertal rhesus monkey. In this study, we characterized the neuron-like nature of these cells and examined distribution and frequency of neuronal elements in the testes of monkeys during postnatal development, puberty and adulthood. Using immunohistochemical methods, we detected both nerve fibers and cell bodies, immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP-25), TH and neuropeptide Y (NPY) in perivascular locations, intermingled with interstitial cells and close to the wall of seminiferous tubules. Marked age-related differences in the numbers of these neuronal elements became apparent, when we quantified NF-200-immunoreactive neuronal elements. Thus, intrinsic neuron-like cell bodies were found only in the testes from immature animals (i.e., until about 3 years of age). Conversely, nerve fibers, presumably representing mainly the extrinsic innervation, were observed at all ages although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, another testicular cell type known to contain potent regulatory substances, mast cells, was found to be in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same pattern as nerve fibers. These results confirm that the testicular nervous system of the monkey is composed of two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components show a marked degree of plasticity during development, especially around the time of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides are likely to have multiple sites of actions, and may affect Leydig cells, cells of the tubular wall and vascular cells directly and/or indirectly via intermediation of mast cells.",
    keywords = "Catecholamines, Gonads, Mast cells, Peripheral neuroendocrinology, Primates, Testis",
    author = "Frungieri, {Monica B.} and Henryk Urbanski and Barbara H{\"o}hne-Zell and Artur Mayerhofer",
    year = "2000",
    language = "English (US)",
    volume = "71",
    pages = "43--50",
    journal = "Neuroendocrinology",
    issn = "0028-3835",
    publisher = "S. Karger AG",
    number = "1",

    }

    TY - JOUR

    T1 - Neuronal elements in the testis of the rhesus monkey

    T2 - Ontogeny, characterization and relationship to testicular cells

    AU - Frungieri, Monica B.

    AU - Urbanski, Henryk

    AU - Höhne-Zell, Barbara

    AU - Mayerhofer, Artur

    PY - 2000

    Y1 - 2000

    N2 - Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) were recently identified in the testis of the prepubertal rhesus monkey. In this study, we characterized the neuron-like nature of these cells and examined distribution and frequency of neuronal elements in the testes of monkeys during postnatal development, puberty and adulthood. Using immunohistochemical methods, we detected both nerve fibers and cell bodies, immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP-25), TH and neuropeptide Y (NPY) in perivascular locations, intermingled with interstitial cells and close to the wall of seminiferous tubules. Marked age-related differences in the numbers of these neuronal elements became apparent, when we quantified NF-200-immunoreactive neuronal elements. Thus, intrinsic neuron-like cell bodies were found only in the testes from immature animals (i.e., until about 3 years of age). Conversely, nerve fibers, presumably representing mainly the extrinsic innervation, were observed at all ages although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, another testicular cell type known to contain potent regulatory substances, mast cells, was found to be in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same pattern as nerve fibers. These results confirm that the testicular nervous system of the monkey is composed of two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components show a marked degree of plasticity during development, especially around the time of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides are likely to have multiple sites of actions, and may affect Leydig cells, cells of the tubular wall and vascular cells directly and/or indirectly via intermediation of mast cells.

    AB - Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) were recently identified in the testis of the prepubertal rhesus monkey. In this study, we characterized the neuron-like nature of these cells and examined distribution and frequency of neuronal elements in the testes of monkeys during postnatal development, puberty and adulthood. Using immunohistochemical methods, we detected both nerve fibers and cell bodies, immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP-25), TH and neuropeptide Y (NPY) in perivascular locations, intermingled with interstitial cells and close to the wall of seminiferous tubules. Marked age-related differences in the numbers of these neuronal elements became apparent, when we quantified NF-200-immunoreactive neuronal elements. Thus, intrinsic neuron-like cell bodies were found only in the testes from immature animals (i.e., until about 3 years of age). Conversely, nerve fibers, presumably representing mainly the extrinsic innervation, were observed at all ages although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, another testicular cell type known to contain potent regulatory substances, mast cells, was found to be in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same pattern as nerve fibers. These results confirm that the testicular nervous system of the monkey is composed of two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components show a marked degree of plasticity during development, especially around the time of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides are likely to have multiple sites of actions, and may affect Leydig cells, cells of the tubular wall and vascular cells directly and/or indirectly via intermediation of mast cells.

    KW - Catecholamines

    KW - Gonads

    KW - Mast cells

    KW - Peripheral neuroendocrinology

    KW - Primates

    KW - Testis

    UR - http://www.scopus.com/inward/record.url?scp=0033957451&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0033957451&partnerID=8YFLogxK

    M3 - Article

    C2 - 10644898

    AN - SCOPUS:0033957451

    VL - 71

    SP - 43

    EP - 50

    JO - Neuroendocrinology

    JF - Neuroendocrinology

    SN - 0028-3835

    IS - 1

    ER -