Neuronal differentiation in cultures of murine neural crest. I. Neurotransmitter expression

Research output: Contribution to journalArticle

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Abstract

This study examines the properties of neurons differentiating in cultures of mammalian neural crest cells. The neurons fall into two categories: (1) a population of early differentiating (ED) neurons generated from precursors that are postmitotic at the time of plating; and (2) a late differentiating (LD) population of neurons arising from dividing precursor cells. The ED population of neurons survive for only 2-3 days while the LD neurons survive for many weeks. Both groups of neurons express the neuronal marker, neurofilament, as well as adrenergic and cholinergic characteristics. The latter two traits are evident as immunoreactivity for tyrosine hydroxylase (TH)/dopamine-β-hydroxylase (DβH) and choline acetyltransferase (ChAT), respectively. The LD neurons also contain immunoreactivity for a number of neuropeptides including, substance P (SP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin gene related polypeptide (CGRP), and somatostatin (SOM). Immunoreactivity for SP, CGRP, and VIP are found in virtually all of the LD neurons while SOM and NPY are found in a smaller percentage of the neurons.

Original languageEnglish (US)
Pages (from-to)1-16
Number of pages16
JournalDevelopmental Brain Research
Volume83
Issue number1
DOIs
StatePublished - Nov 18 1994

Fingerprint

Neural Crest
Neurotransmitter Agents
Neurons
Neuropeptide Y
Vasoactive Intestinal Peptide
Calcitonin
Substance P
Somatostatin
Population
Peptides
Choline O-Acetyltransferase
Intermediate Filaments
Tyrosine 3-Monooxygenase
Mixed Function Oxygenases
Neuropeptides
Adrenergic Agents
Cholinergic Agents
Genes
Dopamine

Keywords

  • Differentiation
  • Immunohistochemistry
  • Neural crest
  • Neurotransmitter
  • Survival

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Neuronal differentiation in cultures of murine neural crest. I. Neurotransmitter expression. / Matsumoto, Steven.

In: Developmental Brain Research, Vol. 83, No. 1, 18.11.1994, p. 1-16.

Research output: Contribution to journalArticle

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