Abstract
This study examines the properties of neurons differentiating in cultures of mammalian neural crest cells. The neurons fall into two categories: (1) a population of early differentiating (ED) neurons generated from precursors that are postmitotic at the time of plating; and (2) a late differentiating (LD) population of neurons arising from dividing precursor cells. The ED population of neurons survive for only 2-3 days while the LD neurons survive for many weeks. Both groups of neurons express the neuronal marker, neurofilament, as well as adrenergic and cholinergic characteristics. The latter two traits are evident as immunoreactivity for tyrosine hydroxylase (TH)/dopamine-β-hydroxylase (DβH) and choline acetyltransferase (ChAT), respectively. The LD neurons also contain immunoreactivity for a number of neuropeptides including, substance P (SP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin gene related polypeptide (CGRP), and somatostatin (SOM). Immunoreactivity for SP, CGRP, and VIP are found in virtually all of the LD neurons while SOM and NPY are found in a smaller percentage of the neurons.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-16 |
| Number of pages | 16 |
| Journal | Developmental Brain Research |
| Volume | 83 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 18 1994 |
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Keywords
- Differentiation
- Immunohistochemistry
- Neural crest
- Neurotransmitter
- Survival
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Neuronal differentiation in cultures of murine neural crest. I. Neurotransmitter expression. / Matsumoto, Steven.
In: Developmental Brain Research, Vol. 83, No. 1, 18.11.1994, p. 1-16.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuronal differentiation in cultures of murine neural crest. I. Neurotransmitter expression
AU - Matsumoto, Steven
PY - 1994/11/18
Y1 - 1994/11/18
N2 - This study examines the properties of neurons differentiating in cultures of mammalian neural crest cells. The neurons fall into two categories: (1) a population of early differentiating (ED) neurons generated from precursors that are postmitotic at the time of plating; and (2) a late differentiating (LD) population of neurons arising from dividing precursor cells. The ED population of neurons survive for only 2-3 days while the LD neurons survive for many weeks. Both groups of neurons express the neuronal marker, neurofilament, as well as adrenergic and cholinergic characteristics. The latter two traits are evident as immunoreactivity for tyrosine hydroxylase (TH)/dopamine-β-hydroxylase (DβH) and choline acetyltransferase (ChAT), respectively. The LD neurons also contain immunoreactivity for a number of neuropeptides including, substance P (SP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin gene related polypeptide (CGRP), and somatostatin (SOM). Immunoreactivity for SP, CGRP, and VIP are found in virtually all of the LD neurons while SOM and NPY are found in a smaller percentage of the neurons.
AB - This study examines the properties of neurons differentiating in cultures of mammalian neural crest cells. The neurons fall into two categories: (1) a population of early differentiating (ED) neurons generated from precursors that are postmitotic at the time of plating; and (2) a late differentiating (LD) population of neurons arising from dividing precursor cells. The ED population of neurons survive for only 2-3 days while the LD neurons survive for many weeks. Both groups of neurons express the neuronal marker, neurofilament, as well as adrenergic and cholinergic characteristics. The latter two traits are evident as immunoreactivity for tyrosine hydroxylase (TH)/dopamine-β-hydroxylase (DβH) and choline acetyltransferase (ChAT), respectively. The LD neurons also contain immunoreactivity for a number of neuropeptides including, substance P (SP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin gene related polypeptide (CGRP), and somatostatin (SOM). Immunoreactivity for SP, CGRP, and VIP are found in virtually all of the LD neurons while SOM and NPY are found in a smaller percentage of the neurons.
KW - Differentiation
KW - Immunohistochemistry
KW - Neural crest
KW - Neurotransmitter
KW - Survival
UR - http://www.scopus.com/inward/record.url?scp=0028063309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028063309&partnerID=8YFLogxK
U2 - 10.1016/0165-3806(94)90174-0
DO - 10.1016/0165-3806(94)90174-0
M3 - Article
C2 - 7697863
AN - SCOPUS:0028063309
VL - 83
SP - 1
EP - 16
JO - Developmental Brain Research
JF - Developmental Brain Research
SN - 0165-3806
IS - 1
ER -