Natural killer cell activation and modulation of chemokine receptor profile in vitro by an extract from the cyanophyta Aphanizomenon flos-aquae

Aaron N. Hart, Lue Ann Zaske, Kelly M. Patterson, Christian Drapeau, Gitte S. Jensen

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The present research was designed to study the effects of an extract from the edible cyanophyta Aphanizomenon flos-aquae on human natural killer (NK) cells. We have previously shown, using a double-blind randomized placebo-controlled crossover design, that ingestion of 1.5 g of dried whole A. flos-aquae resulted in a transient reduction in peripheral blood NK cells in 21 healthy human volunteers, suggesting increased NK cell homing into tissue. We have now identified an extract from A. flos-aquae (AFAe) that directly activates NK cells in vitro and modulates the chemokine receptor profile. NK cell activation was evaluated by expression of CD25 and CD69 on CD3 -CD56+ cells after 18 hours. Changes in CXCR3 and CXCR4 chemokine receptor expression after 5-60 minutes were evaluated by immunostaining and flow cytometry. AFAe induced the expression of CD69 on CD3-CD56+ NK cells, induced CD25 expression on 25% of these cells, and acted in synergy with interleukin 2. NK cells enriched by RosetteSep® (StemCell Technologies Inc., Vancouver, BC, Canada) were not activated by AFAe, indicating that the NK activation was dependent on other cells such as monocytes. The low-molecular-weight fraction <5,000 of AFAe was responsible for the most robust NK cell activation, suggesting novel compounds different from previously reported macrophage-activating large polysaccharides.

Original languageEnglish (US)
Pages (from-to)435-441
Number of pages7
JournalJournal of Medicinal Food
Volume10
Issue number3
DOIs
StatePublished - Sep 2007

Keywords

  • Aphanizomenon flos-aquae
  • Blue-green algae
  • Human
  • Immunomodulation
  • Low-molecular-weight peptides
  • Trafficking

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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