Natural history and histology in a rat model of laser-induced photothrombotic retinal vein occlusion

Yi Zhang, Brad Fortune, La Ongsri Atchaneeyasakul, Trevor McFarland, Kristen Mose, Patrick Wallace, Julianne Main, David Wilson, Binoy Appukuttan, J. Timothy Stout

Research output: Contribution to journalArticle

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Abstract

Purpose: To observe temporal changes in retinal physiology and histology in a rat model of laser-induced retinal vein occlusion (RVO). Methods: Ophthalmoscopy, fundus photography, and fluorescein angiography (FA) were performed following laser-induced central retinal vein occlusion (CRVO) and branch retinal vein occlusion (BRVO) immediately after laser treatment and at 3 and 6 hr and 2, 4, 7, 14, and 21 days. Retinal histology was examined at 4, 7, 14, and 21 days. Full-field electroretinogram was recorded from both eyes simultaneously at day 4. Results: For CRVO and BRVO, reperfusion of occluded branch veins was observed 1 to 2 days after treatment. Despite complete reperfusion of branch veins, retinal edema and hemorrhages peaked on day 4, and by day 14, treated retinas appeared pale and edematous upon ophthalmoscopy. In BRVO animals, retinal hemorrhages were limited to the vein-occluded region, although edema was more widespread and, to a limited extent, involved the untreated hemi-retina. Significant GCL cell loss was observed in both CRVO and BRVO groups after day 14. Regional analysis showed that relative GCL loss was greatest in the peripheral retina in BRVO group. Electroretinography disclosed moderate to severe functional deficits in photoreceptors, bipolar, and amacrine and ganglion cells. Conclusion: Laser-induced RVO in rats results in targeted vascular occlusion that persisted for 1 to 2 days. Functional deficits were evident and significant GCL cell loss was seen, notably within peripheral retina of the BRVO model. This reproducible model provides a valuable tool for the study of the molecular events associated with retinal ischemia and cell death.

Original languageEnglish (US)
Pages (from-to)365-376
Number of pages12
JournalCurrent Eye Research
Volume33
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Retinal Vein Occlusion
Natural History
Histology
Lasers
Retinal Vein
Retina
Retinal Hemorrhage
Ophthalmoscopy
Veins
Reperfusion
Electroretinography
Amacrine Cells
Papilledema
Fluorescein Angiography
Photography
Ganglia
Blood Vessels
Edema
Cell Death
Ischemia

Keywords

  • Electroretinography
  • Histology
  • Ischemia
  • Natural history
  • Retinal venous occlusion

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Zhang, Y., Fortune, B., Atchaneeyasakul, L. O., McFarland, T., Mose, K., Wallace, P., ... Stout, J. T. (2008). Natural history and histology in a rat model of laser-induced photothrombotic retinal vein occlusion. Current Eye Research, 33(4), 365-376. https://doi.org/10.1080/02713680801939318

Natural history and histology in a rat model of laser-induced photothrombotic retinal vein occlusion. / Zhang, Yi; Fortune, Brad; Atchaneeyasakul, La Ongsri; McFarland, Trevor; Mose, Kristen; Wallace, Patrick; Main, Julianne; Wilson, David; Appukuttan, Binoy; Stout, J. Timothy.

In: Current Eye Research, Vol. 33, No. 4, 04.2008, p. 365-376.

Research output: Contribution to journalArticle

Zhang, Y, Fortune, B, Atchaneeyasakul, LO, McFarland, T, Mose, K, Wallace, P, Main, J, Wilson, D, Appukuttan, B & Stout, JT 2008, 'Natural history and histology in a rat model of laser-induced photothrombotic retinal vein occlusion', Current Eye Research, vol. 33, no. 4, pp. 365-376. https://doi.org/10.1080/02713680801939318
Zhang, Yi ; Fortune, Brad ; Atchaneeyasakul, La Ongsri ; McFarland, Trevor ; Mose, Kristen ; Wallace, Patrick ; Main, Julianne ; Wilson, David ; Appukuttan, Binoy ; Stout, J. Timothy. / Natural history and histology in a rat model of laser-induced photothrombotic retinal vein occlusion. In: Current Eye Research. 2008 ; Vol. 33, No. 4. pp. 365-376.
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AU - Mose, Kristen

AU - Wallace, Patrick

AU - Main, Julianne

AU - Wilson, David

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AU - Stout, J. Timothy

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N2 - Purpose: To observe temporal changes in retinal physiology and histology in a rat model of laser-induced retinal vein occlusion (RVO). Methods: Ophthalmoscopy, fundus photography, and fluorescein angiography (FA) were performed following laser-induced central retinal vein occlusion (CRVO) and branch retinal vein occlusion (BRVO) immediately after laser treatment and at 3 and 6 hr and 2, 4, 7, 14, and 21 days. Retinal histology was examined at 4, 7, 14, and 21 days. Full-field electroretinogram was recorded from both eyes simultaneously at day 4. Results: For CRVO and BRVO, reperfusion of occluded branch veins was observed 1 to 2 days after treatment. Despite complete reperfusion of branch veins, retinal edema and hemorrhages peaked on day 4, and by day 14, treated retinas appeared pale and edematous upon ophthalmoscopy. In BRVO animals, retinal hemorrhages were limited to the vein-occluded region, although edema was more widespread and, to a limited extent, involved the untreated hemi-retina. Significant GCL cell loss was observed in both CRVO and BRVO groups after day 14. Regional analysis showed that relative GCL loss was greatest in the peripheral retina in BRVO group. Electroretinography disclosed moderate to severe functional deficits in photoreceptors, bipolar, and amacrine and ganglion cells. Conclusion: Laser-induced RVO in rats results in targeted vascular occlusion that persisted for 1 to 2 days. Functional deficits were evident and significant GCL cell loss was seen, notably within peripheral retina of the BRVO model. This reproducible model provides a valuable tool for the study of the molecular events associated with retinal ischemia and cell death.

AB - Purpose: To observe temporal changes in retinal physiology and histology in a rat model of laser-induced retinal vein occlusion (RVO). Methods: Ophthalmoscopy, fundus photography, and fluorescein angiography (FA) were performed following laser-induced central retinal vein occlusion (CRVO) and branch retinal vein occlusion (BRVO) immediately after laser treatment and at 3 and 6 hr and 2, 4, 7, 14, and 21 days. Retinal histology was examined at 4, 7, 14, and 21 days. Full-field electroretinogram was recorded from both eyes simultaneously at day 4. Results: For CRVO and BRVO, reperfusion of occluded branch veins was observed 1 to 2 days after treatment. Despite complete reperfusion of branch veins, retinal edema and hemorrhages peaked on day 4, and by day 14, treated retinas appeared pale and edematous upon ophthalmoscopy. In BRVO animals, retinal hemorrhages were limited to the vein-occluded region, although edema was more widespread and, to a limited extent, involved the untreated hemi-retina. Significant GCL cell loss was observed in both CRVO and BRVO groups after day 14. Regional analysis showed that relative GCL loss was greatest in the peripheral retina in BRVO group. Electroretinography disclosed moderate to severe functional deficits in photoreceptors, bipolar, and amacrine and ganglion cells. Conclusion: Laser-induced RVO in rats results in targeted vascular occlusion that persisted for 1 to 2 days. Functional deficits were evident and significant GCL cell loss was seen, notably within peripheral retina of the BRVO model. This reproducible model provides a valuable tool for the study of the molecular events associated with retinal ischemia and cell death.

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KW - Ischemia

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KW - Retinal venous occlusion

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