Native species of helix destabilizing protein-1 in mouse myeloma identified by antibody probing of Western blots

Stephen R. Planck, Samuel H. Wilson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Antibody probing of Western blots is a method for analyzing the apparent Mr of a protein in any given preparation (Renart, J., Reiser, J., and Stark, G., Proc. Natl. Acad. Sci. USA 76: 3116, 1979). We prepared a rabbit antiserum to purified mouse myeloma helix destabilizing protein-1 and used this antiserum in Western blotting experiments with a crude homogenate of mouse myeloma. The results indicated that the native species of helix destabilizing protein-1 can be degraded during purification. This in vitro proteolysis results in complete loss of the native species and accumulation of lower Mr proteins that represent limit digestion products. These findings have identified the true native form of mouse myeloma HDP-1 as a protein of apparent Mr=36,000 to 38,000, instead of the Mr=24,000 and 27,000 proteins obtained by routine purification.

Original languageEnglish (US)
Pages (from-to)362-369
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume131
Issue number1
DOIs
StatePublished - Aug 30 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Native species of helix destabilizing protein-1 in mouse myeloma identified by antibody probing of Western blots'. Together they form a unique fingerprint.

  • Cite this