Myosin-dependent targeting of transmembrane proteins to neuronal dendrites

Tommy L. Lewis, Tianyi Mao, Karel Svoboda, Don B. Arnold

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The distinct electrical properties of axonal and dendritic membranes are largely a result of specific transport of vesicle-bound membrane proteins to each compartment. How this specificity arises is unclear because kinesin motors that transport vesicles cannot autonomously distinguish dendritically projecting microtubules from those projecting axonally. We hypothesized that interaction with a second motor might enable vesicles containing dendritic proteins to preferentially associate with dendritically projecting microtubules and avoid those that project to the axon. Here we show that in rat cortical neurons, localization of several distinct transmembrane proteins to dendrites is dependent on specific myosin motors and an intact actin network. Moreover, fusion with a myosin-binding domain from Melanophilin targeted Channelrhodopsin-2 specifically to the somatodendritic compartment of neurons in mice in vivo. Together, our results suggest that dendritic transmembrane proteins direct the vesicles in which they are transported to avoid the axonal compartment through interaction with myosin motors.

Original languageEnglish (US)
Pages (from-to)568-576
Number of pages9
JournalNature Neuroscience
Volume12
Issue number5
DOIs
StatePublished - May 2009
Externally publishedYes

Fingerprint

Protein Transport
Myosins
Dendrites
Transport Vesicles
Microtubules
Neurons
Kinesin
Proteins
Axons
Actins
Membrane Proteins
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Myosin-dependent targeting of transmembrane proteins to neuronal dendrites. / Lewis, Tommy L.; Mao, Tianyi; Svoboda, Karel; Arnold, Don B.

In: Nature Neuroscience, Vol. 12, No. 5, 05.2009, p. 568-576.

Research output: Contribution to journalArticle

Lewis, Tommy L. ; Mao, Tianyi ; Svoboda, Karel ; Arnold, Don B. / Myosin-dependent targeting of transmembrane proteins to neuronal dendrites. In: Nature Neuroscience. 2009 ; Vol. 12, No. 5. pp. 568-576.
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