Myocardial infarction stimulates galanin expression in cardiac sympathetic neurons

Beth Habecker, K. R. Gritman, B. D. Willison, D. M. Van Winkle

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cardiac ischemia-reperfusion alters sympathetic neurotransmission in the heart, but little is known about its effect on neuropeptide expression in sympathetic neurons. Ischemia followed by reperfusion induces the production of inflammatory cytokines in the heart, including interleukin-6 and cardiotrophin-1. These cytokines and related molecules inhibit the expression of neuropeptide Y (NPY), and stimulate the expression of vasoactive intestinal peptide (VIP), substance P (SubP), and galanin (GAL) in cultured sympathetic neurons. Therefore, we quantified NPY, VIP, SubP, and GAL mRNA in neurons of the stellate ganglia 1 week after ischemia-reperfusion to determine if neuropeptide expression was altered in cardiac sympathetic neurons. NPY, VIP, and SubP mRNAs were unchanged compared to unoperated control animals, but GAL mRNA was increased significantly. The increased GAL mRNA was not accompanied by elevated GAL peptide content in the stellate ganglia. Galanin content was increased significantly in the heart, however, indicating that elevated GAL mRNA led to increased peptide production. GAL content was increased in the left ventricle below the coronary artery ligation, but was not increased significantly in the atria or the base of the heart above the ligation. The buildup of GAL specifically in the damaged left ventricle is consistent with previous reports that GAL is transported to regenerating nerve endings after axon damage.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalNeuropeptides
Volume39
Issue number2
DOIs
StatePublished - Apr 2005

Fingerprint

Galanin
Neurons
Myocardial Infarction
Neuropeptide Y
Vasoactive Intestinal Peptide
Substance P
Messenger RNA
Reperfusion
Stellate Ganglion
Ischemia
Neuropeptides
Heart Ventricles
Ligation
Cytokines
Peptides
Nerve Endings
Synaptic Transmission
Axons
Interleukin-6
Coronary Vessels

Keywords

  • Axotomy
  • Galanin
  • Neuropeptide Y
  • Substance P
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Neurology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Biochemistry

Cite this

Myocardial infarction stimulates galanin expression in cardiac sympathetic neurons. / Habecker, Beth; Gritman, K. R.; Willison, B. D.; Van Winkle, D. M.

In: Neuropeptides, Vol. 39, No. 2, 04.2005, p. 89-95.

Research output: Contribution to journalArticle

Habecker, Beth ; Gritman, K. R. ; Willison, B. D. ; Van Winkle, D. M. / Myocardial infarction stimulates galanin expression in cardiac sympathetic neurons. In: Neuropeptides. 2005 ; Vol. 39, No. 2. pp. 89-95.
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