MYC regulates ductal-neuroendocrine lineage plasticity in pancreatic ductal adenocarcinoma associated with poor outcome and chemoresistance

Amy Farrell, Meghan Morrison Joly, Brittany L. Allen-Petersen, Patrick J. Worth, Christian Lanciault, David Sauer, Jason Link, Carl Pelz, Laura Heiser, Jennifer P. Morton, Nathiya Muthalagu, Megan T. Hoffman, Sara L. Manning, Erica D. Pratt, Nicholas D. Kendsersky, Nkolika Egbukichi, Taylor S. Amery, Mary C. Thoma, Zina P. Jenny, Andrew D. RhimDaniel J. Murphy, Owen J. Sansom, Howard C. Crawford, Brett Sheppard, Rosalie Sears

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Intratumoral phenotypic heterogeneity has been described in many tumor types, where it can contribute to drug resistance and disease recurrence. We analyzed ductal and neuroendocrine markers in pancreatic ductal adenocarcinoma, revealing heterogeneous expression of the neuroendocrine marker Synaptophysin within ductal lesions. Higher percentages of Cytokeratin-Synaptophysin dual positive tumor cells correlate with shortened disease-free survival. We observe similar lineage marker heterogeneity in mouse models of pancreatic ductal adenocarcinoma, where lineage tracing indicates that Cytokeratin-Synaptophysin dual positive cells arise from the exocrine compartment. Mechanistically, MYC binding is enriched at neuroendocrine genes in mouse tumor cells and loss of MYC reduces ductal-neuroendocrine lineage heterogeneity, while deregulated MYC expression in KRAS mutant mice increases this phenotype. Neuroendocrine marker expression is associated with chemoresistance and reducing MYC levels decreases gemcitabine-induced neuroendocrine marker expression and increases chemosensitivity. Altogether, we demonstrate that MYC facilitates ductal-neuroendocrine lineage plasticity in pancreatic ductal adenocarcinoma, contributing to poor survival and chemoresistance.

Original languageEnglish (US)
Article number1728
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

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Synaptophysin
plastic properties
markers
Plasticity
Tumors
Adenocarcinoma
Cells
gemcitabine
Keratins
mice
tumors
Neoplasms
Drug Resistance
Disease-Free Survival
Genes
phenotype
Phenotype
Recurrence
compartments
tracing

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

MYC regulates ductal-neuroendocrine lineage plasticity in pancreatic ductal adenocarcinoma associated with poor outcome and chemoresistance. / Farrell, Amy; Joly, Meghan Morrison; Allen-Petersen, Brittany L.; Worth, Patrick J.; Lanciault, Christian; Sauer, David; Link, Jason; Pelz, Carl; Heiser, Laura; Morton, Jennifer P.; Muthalagu, Nathiya; Hoffman, Megan T.; Manning, Sara L.; Pratt, Erica D.; Kendsersky, Nicholas D.; Egbukichi, Nkolika; Amery, Taylor S.; Thoma, Mary C.; Jenny, Zina P.; Rhim, Andrew D.; Murphy, Daniel J.; Sansom, Owen J.; Crawford, Howard C.; Sheppard, Brett; Sears, Rosalie.

In: Nature Communications, Vol. 8, No. 1, 1728, 01.12.2017.

Research output: Contribution to journalArticle

Farrell, A, Joly, MM, Allen-Petersen, BL, Worth, PJ, Lanciault, C, Sauer, D, Link, J, Pelz, C, Heiser, L, Morton, JP, Muthalagu, N, Hoffman, MT, Manning, SL, Pratt, ED, Kendsersky, ND, Egbukichi, N, Amery, TS, Thoma, MC, Jenny, ZP, Rhim, AD, Murphy, DJ, Sansom, OJ, Crawford, HC, Sheppard, B & Sears, R 2017, 'MYC regulates ductal-neuroendocrine lineage plasticity in pancreatic ductal adenocarcinoma associated with poor outcome and chemoresistance', Nature Communications, vol. 8, no. 1, 1728. https://doi.org/10.1038/s41467-017-01967-6
Farrell, Amy ; Joly, Meghan Morrison ; Allen-Petersen, Brittany L. ; Worth, Patrick J. ; Lanciault, Christian ; Sauer, David ; Link, Jason ; Pelz, Carl ; Heiser, Laura ; Morton, Jennifer P. ; Muthalagu, Nathiya ; Hoffman, Megan T. ; Manning, Sara L. ; Pratt, Erica D. ; Kendsersky, Nicholas D. ; Egbukichi, Nkolika ; Amery, Taylor S. ; Thoma, Mary C. ; Jenny, Zina P. ; Rhim, Andrew D. ; Murphy, Daniel J. ; Sansom, Owen J. ; Crawford, Howard C. ; Sheppard, Brett ; Sears, Rosalie. / MYC regulates ductal-neuroendocrine lineage plasticity in pancreatic ductal adenocarcinoma associated with poor outcome and chemoresistance. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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AU - Sears, Rosalie

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