Mutation of luxS affects biofilm formation in Streptococcus mutans

Justin Merritt, Fengxia Qi, Steven D. Goodman, Maxwell H. Anderson, Wenyuan Shi

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Quorum sensing is a bacterial mechanism for regulating gene expression in response to changes in population density. Many bacteria are capable of acyl-homoserine lactone-based or peptide-based intraspecies quorum sensing and luxS-dependent interspecies quorum sensing. While there is good evidence about the involvement of intraspecies quorum sensing in bacterial biofilm, little is known about the role of luxS in biofilm formation. In this study, we report for the first time that luxS-dependent quorum sensing is involved in biofilm formation of Streptococcus mutans. S. mutans is a major cariogenic bacterium in the multispecies bacterial biofilm commonly known as dental plaque. An ortholog of luxS for S. mutans was identified using the data available in the S. mutans genome project (http://www.genome.ou.edu/smutans.html). Using an assay developed for the detection of the LuxS-associated quorum sensing signal autoinducer 2 (AI-2), it was demonstrated that this ortholog was able to complement the luxS negative phenotype of Escherichia coli DH5α. It was also shown that AI-2 is indeed produced by S. mutans. AI-2 production is maximal during mid- to late-log growth in batch culture. Mutant strains devoid of the luxS gene were constructed and found to be defective in producing the AI-2 signal. There are also marked phenotypic differences between the wild type and the luxS mutants. Microscopic analysis of in vitro-grown biofilm structure revealed that the luxS mutant biofilms adopted a much more granular appearance, rather than the relatively smooth, confluent layer normally seen in the wild type. These results suggest that LuxS-dependent signal may play an important role in biofilm formation of S. mutans.

Original languageEnglish (US)
Pages (from-to)1972-1979
Number of pages8
JournalInfection and Immunity
Volume71
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Streptococcus mutans
Quorum Sensing
Biofilms
Mutation
Acyl-Butyrolactones
Genome
Bacteria
Dental Plaque
Batch Cell Culture Techniques
Population Density
Escherichia coli
Phenotype
Gene Expression
Peptides
N-octanoylhomoserine lactone
Growth
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Mutation of luxS affects biofilm formation in Streptococcus mutans. / Merritt, Justin; Qi, Fengxia; Goodman, Steven D.; Anderson, Maxwell H.; Shi, Wenyuan.

In: Infection and Immunity, Vol. 71, No. 4, 01.04.2003, p. 1972-1979.

Research output: Contribution to journalArticle

Merritt, Justin ; Qi, Fengxia ; Goodman, Steven D. ; Anderson, Maxwell H. ; Shi, Wenyuan. / Mutation of luxS affects biofilm formation in Streptococcus mutans. In: Infection and Immunity. 2003 ; Vol. 71, No. 4. pp. 1972-1979.
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