Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging

Pengfei Zhang, Azhar Zam, Yifan Jian, Xinlei Wang, Marie E. Burns, Marinko V. Sarunic, Edward N. Pugh, Robert J. Zawadzki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A compact, non-invasive multi-modal system has been developed for in vivo mouse retina imaging. It is configured for simultaneously detecting green and red fluorescent protein signals with scanning laser ophthalmoscopy (SLO) back-scattered light from the SLO illumination beam, and depth information about different retinal layers by means of Optical Coherence Tomography (OCT). Simultaneous assessment of retinal characteristics with different modalities can provide a wealth of information about the structural and functional changes in the retinal neural tissue and chorio-retinal vasculature in vivo. Additionally, simultaneous acquisition of multiple channels facilitates analysis of the data of different modalities by automatic temporal and structural co-registration. As an example of the instrument’s performance we imaged the retina of a mouse with constitutive expression of GFP in microglia cells (Cx3cr1GFP/+), and which also expressed the red fluorescent protein mCherry in Müller glial cells by means of adeno-Associated virus delivery (AAV2) of an mCherry cDNA driven by the GFAP (glial fibrillary acid protein) promoter.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XXV
EditorsArthur Ho, Fabrice Manns, Per G. Soderberg
PublisherSPIE
ISBN (Electronic)9781628413977
DOIs
StatePublished - Jan 1 2015
Externally publishedYes
Event25th Conference on Ophthalmic Technologies - San Francisco, United States
Duration: Feb 7 2015Feb 8 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9307
ISSN (Print)1605-7422

Conference

Conference25th Conference on Ophthalmic Technologies
CountryUnited States
CitySan Francisco
Period2/7/152/8/15

Fingerprint

Ophthalmoscopy
Optical tomography
Optical Coherence Tomography
mice
Retina
Lasers
retina
tomography
proteins
Proteins
Scanning
Imaging techniques
Dependovirus
scanning
Glial Fibrillary Acidic Protein
Microglia
Green Fluorescent Proteins
Lighting
Viruses
Neuroglia

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Zhang, P., Zam, A., Jian, Y., Wang, X., Burns, M. E., Sarunic, M. V., ... Zawadzki, R. J. (2015). Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging. In A. Ho, F. Manns, & P. G. Soderberg (Eds.), Ophthalmic Technologies XXV [93070H] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9307). SPIE. https://doi.org/10.1117/12.2080468

Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging. / Zhang, Pengfei; Zam, Azhar; Jian, Yifan; Wang, Xinlei; Burns, Marie E.; Sarunic, Marinko V.; Pugh, Edward N.; Zawadzki, Robert J.

Ophthalmic Technologies XXV. ed. / Arthur Ho; Fabrice Manns; Per G. Soderberg. SPIE, 2015. 93070H (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9307).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, P, Zam, A, Jian, Y, Wang, X, Burns, ME, Sarunic, MV, Pugh, EN & Zawadzki, RJ 2015, Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging. in A Ho, F Manns & PG Soderberg (eds), Ophthalmic Technologies XXV., 93070H, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9307, SPIE, 25th Conference on Ophthalmic Technologies, San Francisco, United States, 2/7/15. https://doi.org/10.1117/12.2080468
Zhang P, Zam A, Jian Y, Wang X, Burns ME, Sarunic MV et al. Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging. In Ho A, Manns F, Soderberg PG, editors, Ophthalmic Technologies XXV. SPIE. 2015. 93070H. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2080468
Zhang, Pengfei ; Zam, Azhar ; Jian, Yifan ; Wang, Xinlei ; Burns, Marie E. ; Sarunic, Marinko V. ; Pugh, Edward N. ; Zawadzki, Robert J. / Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging. Ophthalmic Technologies XXV. editor / Arthur Ho ; Fabrice Manns ; Per G. Soderberg. SPIE, 2015. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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