Multiple roles of rnase y in streptococcus pyogenes mrna processing and degradation

Zhiyun Chen, Andreas Itzek, Horst Malke, Joseph J. Ferretti, Jens Kreth

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Control over mRNA stability is an essential part of gene regulation that involves both endo- and exoribonucleases. RNase Y is a recently identified endoribonuclease in Gram-positive bacteria, and an RNase Y ortholog has been identified in Streptococcus pyogenes (group A streptococcus [GAS]). In this study, we used microarray and Northern blot analyses to determine the S. pyogenes mRNA half-life of the transcriptome and to understand the role of RNase Y in global mRNA degradation and processing. We demonstrated that S. pyogenes has an unusually high mRNA turnover rate, with median and mean half-lives of 0.88 min and 1.26 min, respectively. A mutation of the RNase Y-encoding gene (rny) led to a 2-fold increase in overall mRNA stability. RNase Y was also found to play a significant role in the mRNA processing of virulence-associated genes as well as in the rapid degradation of rnpB read-through transcripts. From these results, we conclude that RNase Y is a pleiotropic regulator required for mRNA stability, mRNA processing, and removal of read-through transcripts in S. pyogenes.

Original languageEnglish (US)
Pages (from-to)2585-2594
Number of pages10
JournalJournal of Bacteriology
Volume195
Issue number11
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

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Streptococcus pyogenes
Ribonucleases
RNA Stability
Messenger RNA
Exoribonucleases
Endoribonucleases
Endonucleases
Essential Genes
Gram-Positive Bacteria
Streptococcus
Transcriptome
Northern Blotting
Genes
Half-Life
Virulence
Mutation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Multiple roles of rnase y in streptococcus pyogenes mrna processing and degradation. / Chen, Zhiyun; Itzek, Andreas; Malke, Horst; Ferretti, Joseph J.; Kreth, Jens.

In: Journal of Bacteriology, Vol. 195, No. 11, 01.06.2013, p. 2585-2594.

Research output: Contribution to journalArticle

Chen, Zhiyun ; Itzek, Andreas ; Malke, Horst ; Ferretti, Joseph J. ; Kreth, Jens. / Multiple roles of rnase y in streptococcus pyogenes mrna processing and degradation. In: Journal of Bacteriology. 2013 ; Vol. 195, No. 11. pp. 2585-2594.
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