Multiomics analyses of vesicular transport pathway-specific transcripts and proteins in ovine amnion

responses to altered intramembranous transport

Cecilia Cheung, Debra Anderson, Robert A. Brace

Research output: Contribution to journalArticle

Abstract

Amniotic fluid volume (AFV) is determined by the rate of intramembranous (IM) transport of amniotic fluid (AF) across the amnion. This transport is regulated by fetal urine-derived stimulators and AF inhibitors. Our objective was to utilize a multiomics approach to determine the IM transport pathways and identify the regulators. Four groups of fetal sheep with experimentally induced alterations in IM transport rate were studied: control, urine drainage (UD), urine drainage with fluid replacement (UDR), and intra-amniotic fluid infusion (IA). Amnion, AF, and fetal urine were subjected to transcriptomics (RNA-Seq) and proteomics studies followed by Ingenuity Pathway Analysis. The analysis uncovered nine transport-associated pathways and four groups of differentially expressed transcripts and proteins. These can be categorized into mediators of vesicular uptake and endocytosis, intracellular trafficking, pathway activation and signaling, and energy metabolism. UD decreased IM transport rate and AFV in conjunction with enhanced expression of vesicular endocytosis regulators but reduced expression of intracellular trafficking mediators. With UDR, IM transport rate decreased and AFV increased. Energy metabolism activators increased while trafficking mediators decreased in expression. IA increased IM transport rate and AFV together with enhanced expressions of vesicular endocytosis and trafficking mediators. We conclude that IM transport across the amnion is regulated by multiple vesicular transcytotic and signaling pathways and that the mediators of intracellular trafficking most likely play an important role in determining the rate of IM transport. Furthermore, the motor protein cytoplasmic dynein light chain-1, which coexpressed in AF and fetal urine, may function as a urine-derived IM transport stimulator.

Original languageEnglish (US)
Pages (from-to)267-278
Number of pages12
JournalPhysiological genomics
Volume51
Issue number7
DOIs
StatePublished - Jul 1 2019

Fingerprint

Amnion
Amniotic Fluid
Sheep
Urine
Proteins
Endocytosis
Drainage
Energy Metabolism
Cytoplasmic Dyneins
Transcytosis
Dyneins
Proteomics
RNA

Keywords

  • amniotic fluid volume
  • intramembranous absorption
  • proteomics
  • transcriptomics
  • vesicular transport

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Multiomics analyses of vesicular transport pathway-specific transcripts and proteins in ovine amnion : responses to altered intramembranous transport. / Cheung, Cecilia; Anderson, Debra; Brace, Robert A.

In: Physiological genomics, Vol. 51, No. 7, 01.07.2019, p. 267-278.

Research output: Contribution to journalArticle

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