Multilayer BioDerivatized nanoparticle composites by electric field directed self-assembly

Michael J. Heller, Alexander P. Haiso

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

An electronic microarray has been used to carry out directed self-assembly of higher order 3D structures from biotin/streptavidin, DNA and enzyme derivatized nanoparticles. Structures with up to fifty layers of alternating biotin and streptavidin, DNA and enzyme nanoparticles were fabricated using a 400 site CMOS microarray system. In this process, reconfigurable electric fields produced by the microarray were used to rapidly transport, concentrate and accelerate the binding of 40 and/or 200nm nanometer bio-derivatized nanoparticles to selected sites on the microarray. The nanoparticle layering process takes less than one minute per layer. The nanoparticle addressing/binding process was monitored by changes in fluorescence intensity as each nanoparticle layer was deposited. The final multilayered 3-D structures are about two microns in thickness and 50 microns in diameter. Most recently we have successfully fabricated enzyme-nanoparticle layers with streptavidin-alkaline phosphatase, glucose oxidase-avidin, and streptavidin-HRP. Up to 47 layers were addressed with 200nm nanoparticles and enzyme activity was retained in the assembled structure. This work represents a unique example of combining "top-down" and "bottom-up" technologies into a novel nanofabrication process. Such a process will be useful for the hierarchal assembly of 3D nano, micro, and macrostructures for a variety of electronic/photonic, nanomaterials, energy (photovoltaics, fuel cells, batteries) and biosensor applications.

Original languageEnglish (US)
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages250-253
Number of pages4
StatePublished - Nov 9 2010
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Publication series

NameNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Volume3

Conference

ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
CountryUnited States
CityAnaheim, CA
Period6/21/106/24/10

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Keywords

  • Composites
  • Electric field
  • Higher order structures
  • Nanofabrication
  • Nanoparticles
  • Self-assembly

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

Heller, M. J., & Haiso, A. P. (2010). Multilayer BioDerivatized nanoparticle composites by electric field directed self-assembly. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (pp. 250-253). (Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010; Vol. 3).