Multi-sialo brain gangliosides are powerful stimulators of active E-rosetting lymphocytes from multiple sclerosis patients

Halina Offner, Gregory Konat, Ben Ami Sela

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Peripheral blood T lymphocytes from all of 14 patients with clinically definite multiple sclerosis (MS) were significantly stimulated by MS brain gangliosides in the active rosetting of sheep erythrocytes. Fractionated mono- and disialogangliosides were devoid of any stimulating effect on MS lymphocytes whereas the trisialoganglioside GT1 and to a greater extent the tetrasialoganglioside GQ1b were fully effective at a dose as low as 2 × 10-18 moles. Gangliosides extracted from MS brains or from MS brain myelin were far more effective than gangliosides derived from control human brains or from bovine and mouse brains, suggesting the importance of highly sialylated gangliosides occurring to a greater extent in MS brain as previously reported. Lymphocytes from only 3 out of 24 other neurological patients were stimulated by the slow migrating gangliosides in the same way, but none of 32 healthy subjects responded to these gangliosides in the active E-rosette test. Lymphocytes from 5 of 8 patients with unilateral optic neuritis reacted positively to brain gangliosides by rosette formation, several weeks before a similar reaction to myelin basic protein was evident. Our data are compatible with a release of gangliosides during demyelination or other CNS degenerative processes occurring in multiple sclerosis.

Original languageEnglish (US)
Pages (from-to)279-287
Number of pages9
JournalJournal of the Neurological Sciences
Volume52
Issue number2-3
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Gangliosides
Multiple Sclerosis
Lymphocytes
Brain
Rosette Formation
Optic Neuritis
Myelin Basic Protein
Demyelinating Diseases
Myelin Sheath
Sheep
Healthy Volunteers
Erythrocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

Cite this

Multi-sialo brain gangliosides are powerful stimulators of active E-rosetting lymphocytes from multiple sclerosis patients. / Offner, Halina; Konat, Gregory; Sela, Ben Ami.

In: Journal of the Neurological Sciences, Vol. 52, No. 2-3, 1981, p. 279-287.

Research output: Contribution to journalArticle

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