MsGC-II, a receptor guanylyl cyclase isolated from the CNS of Manduca sexta that is inhibited by calcium

David Morton, Alan Nighorn

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We describe the cloning of a receptor guanylyl cyclase, MsGC-II, from the CNS of the insect Manduca sexta. Sequence comparisons with other receptor guanylyl cyclases show that MsGC-II is most similar to a predicted guanylyl cyclase in the Drosophila genome and to vertebrate retinal guanylyl cyclases. When expressed in COS-7 cells, MsGC-II exhibited a low level of basal activity that was nearly abolished in the presence of 10 μM calcium. Incubation with either a mammalian guanylyl cyclase-activating protein or Drosophila frequenin resulted in only mild stimulation of activity, whereas incubation of COS-7 cells expressing MsGC-II with a variety of Manduca tissue extracts failed to stimulate enzyme activity above basal levels. Analysis of the tissue distribution of MsGC-II revealed that it is nervous system specific. In the adult, MsGC-II is present in neurons in the optic lobes, antennal lobes and cellular cortex, but it is most highly expressed in subsets of intrinsic mushroom body neurons. Thus, MsGC-II appears to be a neural-specific receptor guanylyl cyclase whose activity may be regulated either directly or indirectly by calcium.

Original languageEnglish (US)
Pages (from-to)363-372
Number of pages10
JournalJournal of Neurochemistry
Volume84
Issue number2
DOIs
StatePublished - Jan 2003

Fingerprint

Guanylate Cyclase-Coupled Receptors
Manduca
Guanylate Cyclase
COS Cells
Calcium
Drosophila
Neurons
Guanylate Cyclase-Activating Proteins
Mushroom Bodies
Tissue Extracts
Cloning
Enzyme activity
Neurology
Sensory Receptor Cells
Tissue Distribution
Nervous System
Insects
Vertebrates
Organism Cloning
Optics

Keywords

  • Calcium
  • Cyclic GMP
  • Guanylyl cyclase
  • Neuronal calcium sensor

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

MsGC-II, a receptor guanylyl cyclase isolated from the CNS of Manduca sexta that is inhibited by calcium. / Morton, David; Nighorn, Alan.

In: Journal of Neurochemistry, Vol. 84, No. 2, 01.2003, p. 363-372.

Research output: Contribution to journalArticle

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