MR of carcinoma-specific monoclonal antibody conjugated to monocrystalline iron oxide nanoparticles: The potential for noninvasive diagnosis

Laura G. Remsen, Christopher I. McCormick, Simon Roman-Goldstein, Gajanan Nilaver, Ralph Weissleder, Alexei Bogdanov, Karl E. Hellström, Ingegerd Hellström, Robert A. Kroll, Edward A. Neuwelt

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

PURPOSE: To determine if tumor-specific monoclonal antibodies conjugated to superparamagnetic monocrystalline iron oxide nanoparticles can be used to yield specific diagnoses with the use of MR imaging. METHODS: Monoclonal antibodies conjugated to monocrystalline iron oxide nanoparticles were given to nude rats with intracranial tumors either by intravenous injection, intraarterial injection with osmotic blood-brain barrier disruption, or direct intratumoral inoculation. Either L6, a tumor-specific antibody, or P- 1.17, a control isotype-matched antibody, was used. Coronal T1-weighted, T2- weighted, and spoiled gradient-recalled acquisition in the steady state images were obtained before, 30 minutes after, 6 hours after, and 24 hours after injection. RESULTS: intravenous injection of greater than 2 mg of the tumor-specific antibody showed a specific pattern of enhancement of the tumors with the largest concentration of antibody in the area with the greatest density of tumor cells. The control antibody showed nonspecific changes. After intraarterial injection with barrier disruption to increase delivery globally or direct inoculation to increase delivery focally, no specific enhancement pattern was seen. CONCLUSION: Monoclonal antibodies conjugated with monocrystalline iron oxide particles may provide a method to obtain specific diagnoses with the use of MR imaging.

Original languageEnglish (US)
Pages (from-to)411-418
Number of pages8
JournalAmerican Journal of Neuroradiology
Volume17
Issue number3
StatePublished - 1996

Keywords

  • Animal studies
  • Antigens and antibodies
  • Brain neoplasms, magnetic resonance
  • Carcinoma

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Clinical Neurology

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