Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryos

Shoukhrat Mitalipov, Richard R. Yeoman, Hung Chih Kuo, Don P. Wolf

    Research output: Contribution to journalArticle

    56 Citations (Scopus)

    Abstract

    The nonhuman primate is a relevant model for human disease that can be used for diverse biomedical investigations. The ability to propagate a founder animal by application of assisted reproductive technologies is pressing, but an even greater need in many studies is access to genetically identical animals. In an effort to create genetically identical monkeys, we evaluated two approaches to monozygotic twinning; blastomere separation, and blastocyst bisection. Embryos were produced by intracytoplasmic sperm injection of oocytes recovered following controlled ovarian stimulation. The quality of demiembryos produced in these efforts was evaluated by quantitating the efficiency of creating identical pairs for embryo transfer, by morphological assessment, by the allocation of cells to the inner cell mass (ICM) and trophectoderm (TE) in the blastocyst, and by the outcome of embryo transfer to synchronized host animals. Pairs were produced in high yield (85%-95%) by both twinning methods. Demiembryos resulting from blastomere separations at the 2- or 4-cell stage grew to blastocysts at the control frequency. Demiblastocysts contained, on average, half the number of cells of the intact controls while maintaining the same ICM:TE or ICM:total cell ratio. The equivalency of demiblastocysts within a set was also evaluated by differential cell counting. Embryo transfers of identical sets led to a 33% clinical pregnancy rate, with two twin pregnancies initiated. Neither pregnancy resulted in term birth of monozygotic twins, but our results are sufficiently encouraging to justify a large-scale twinning trial in the rhesus macaque.

    Original languageEnglish (US)
    Pages (from-to)1449-1455
    Number of pages7
    JournalBiology of Reproduction
    Volume66
    Issue number5
    StatePublished - 2002

    Fingerprint

    Monozygotic Twinning
    Macaca mulatta
    Embryonic Structures
    Embryo Transfer
    Blastocyst
    Blastomeres
    Term Birth
    Assisted Reproductive Techniques
    Twin Pregnancy
    Intracytoplasmic Sperm Injections
    Monozygotic Twins
    Ovulation Induction
    Pregnancy Rate
    In Vitro Techniques
    Primates
    Oocytes
    Haplorhini
    Cell Count
    Pregnancy

    Keywords

    • Assisted reproductive technology
    • Embryo
    • Fertilization
    • In vitro fertilization
    • Pregnancy

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryos. / Mitalipov, Shoukhrat; Yeoman, Richard R.; Kuo, Hung Chih; Wolf, Don P.

    In: Biology of Reproduction, Vol. 66, No. 5, 2002, p. 1449-1455.

    Research output: Contribution to journalArticle

    Mitalipov, Shoukhrat ; Yeoman, Richard R. ; Kuo, Hung Chih ; Wolf, Don P. / Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryos. In: Biology of Reproduction. 2002 ; Vol. 66, No. 5. pp. 1449-1455.
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