Molecular cloning and characterization of CAPER, a novel coactivator of activating protein-1 and estrogen receptors

Dong Ju Jung, Soon Young Na, Doe Sun Na, Jae Lee

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Transcriptional coactivators either bridge transcription factors and the components of the basal transcription apparatus and/or remodel the chromatin structures. We isolated a novel nuclear protein based on its interaction with the recently described general coactivator activating signal cointegrator-2 (ASC-2). This protein CAPER (for coactivator of activating protein-1 (AP-1) and estrogen receptors (ERs)) selectively bound, among the many transcription factors we tested, the AP-1 component c-Jun and the estradiol-bound ligand binding domains of ERα and ERβ. Interestingly, CAPER exhibited a cryptic autonomous transactivation function that becomes activated only in the presence of estradiol-bound ER. In cotransfections, CAPER stimulated transactivation by ERα, ERβ, and AP-1. Thus, CAPER may represent a more selective transcriptional coactivator molecule that plays a pivotal role for the function of AP-1 and ERs in vivo in conjunction with the general coactivator ASC-2.

Original languageEnglish (US)
Pages (from-to)1229-1234
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number2
DOIs
StatePublished - Jan 11 2002
Externally publishedYes

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Cloning
Molecular Cloning
Estrogen Receptors
Transcriptional Activation
Estradiol
Proteins
Activating Transcription Factors
Nuclear Proteins
Chromatin
Transcription Factors
Ligands
Transcription
estrophilin
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and characterization of CAPER, a novel coactivator of activating protein-1 and estrogen receptors. / Jung, Dong Ju; Na, Soon Young; Na, Doe Sun; Lee, Jae.

In: Journal of Biological Chemistry, Vol. 277, No. 2, 11.01.2002, p. 1229-1234.

Research output: Contribution to journalArticle

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