Molecular chaperone function for myocilin

Ann Marie Anderssohn, Kalani Cox, Kevin O'Malley, Scott Dees, Mojgan Hosseini, Lacey Boren, Anthony Wagner, John M. Bradley, Mary Kelley, Ted Acott

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose. Myocilin is thought to be a stress response protein, but its exact molecular functions have not been established. Studies were conducted to see whether myocilin can act as a general molecular chaperone. Methods. Myocilin was isolated and purified from porcine trabecular meshwork (TM) cell culture media. Its ability to protect citrate synthase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the restriction endonuclease DrdI from thermal inactivation was evaluated. Light scattering was used to evaluate thermally induced aggregation of citrate synthase. Myocilin induction was assessed after exposure of TM cells to several types of stress treatments. Results. Levels of extracellular myocilin expressed by TM cells were increased in response to mechanical stretch, heat shock, TNFα, or IL-1α. Myocilin protected citrate synthase activity against thermal inactivation for 5 minutes at 55°C in a concentration-dependent manner, with nearly full protection of 1.5 μM citrate synthase in the presence of 650 nM myocilin. Myocilin significantly reduced thermal aggregation of citrate synthase to levels 36% to 44% of control levels. Myocilin also protected GAPDH from thermal inactivation for 10 minutes at 45°C. Myocilin at 18 nM was more effective than 1 μM bovine serum albumin at protecting DrdI from thermal inactivation. Conclusions. Myocilin is induced in response to several cellular stresses and displays general molecular chaperone activity by protecting DrdI, citrate synthase, and GAPDH from thermal inactivation. Myocilin also suppresses the thermal aggregation of citrate synthase. One function of myocilin may be to serve as a molecular chaperone.

Original languageEnglish (US)
Pages (from-to)7548-7555
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number10
DOIs
StatePublished - Sep 2011

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Molecular Chaperones
Citrate (si)-Synthase
Hot Temperature
Trabecular Meshwork
Glyceraldehyde-3-Phosphate Dehydrogenases
trabecular meshwork-induced glucocorticoid response protein
DNA Restriction Enzymes
Bovine Serum Albumin
Heat-Shock Proteins
Interleukin-1
Culture Media
Shock
Swine

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Anderssohn, A. M., Cox, K., O'Malley, K., Dees, S., Hosseini, M., Boren, L., ... Acott, T. (2011). Molecular chaperone function for myocilin. Investigative Ophthalmology and Visual Science, 52(10), 7548-7555. https://doi.org/10.1167/iovs.11-7723

Molecular chaperone function for myocilin. / Anderssohn, Ann Marie; Cox, Kalani; O'Malley, Kevin; Dees, Scott; Hosseini, Mojgan; Boren, Lacey; Wagner, Anthony; Bradley, John M.; Kelley, Mary; Acott, Ted.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 10, 09.2011, p. 7548-7555.

Research output: Contribution to journalArticle

Anderssohn, AM, Cox, K, O'Malley, K, Dees, S, Hosseini, M, Boren, L, Wagner, A, Bradley, JM, Kelley, M & Acott, T 2011, 'Molecular chaperone function for myocilin', Investigative Ophthalmology and Visual Science, vol. 52, no. 10, pp. 7548-7555. https://doi.org/10.1167/iovs.11-7723
Anderssohn AM, Cox K, O'Malley K, Dees S, Hosseini M, Boren L et al. Molecular chaperone function for myocilin. Investigative Ophthalmology and Visual Science. 2011 Sep;52(10):7548-7555. https://doi.org/10.1167/iovs.11-7723
Anderssohn, Ann Marie ; Cox, Kalani ; O'Malley, Kevin ; Dees, Scott ; Hosseini, Mojgan ; Boren, Lacey ; Wagner, Anthony ; Bradley, John M. ; Kelley, Mary ; Acott, Ted. / Molecular chaperone function for myocilin. In: Investigative Ophthalmology and Visual Science. 2011 ; Vol. 52, No. 10. pp. 7548-7555.
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AU - Cox, Kalani

AU - O'Malley, Kevin

AU - Dees, Scott

AU - Hosseini, Mojgan

AU - Boren, Lacey

AU - Wagner, Anthony

AU - Bradley, John M.

AU - Kelley, Mary

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