Molecular approaches to the identification and treatment monitoring of periodontal pathogens

P. Caposio, I. Torta, F. Romano, M. Aimetti, R. Romagnoli, G. Marchiaro, D. M. Cirillo

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Two different PCR-based molecular approaches, a commercial kit for detection of A. actinomycetemcomitans, P. gingivalis, P. intermedia, B. forsythus and T. denticola (Amplimedical "Paradonthosis") and a home-made multiplex PCR for A. actinomycetemcomitans, P. gingivalis and B. forsythus were compared for monitoring the efficacy of different dental treatments on localized persistent periodontal pockets. 44 sites were randomized in two treatment groups: mechanical treatment (22 control sites) and in conjunction with the application of tetracycline fibres (22 experimental sites). 40/44 sites were found positive with both tests for A. actinomycetemcomitans, P. gingivalis and B. forsythus pretheraphy. F. intermedia was detected alone in only three sites during the follow-up, while T. denticola. was always associated with the other pathogens. 20 sites were positive in conventional cultures for one to three of the pathogens. PCR-based approaches provided a sensitive and reliable method for identification and monitoring treatment of periodontal pathogens.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalNew Microbiologica
Volume26
Issue number1
StatePublished - Jan 1 2003

Keywords

  • Line probe assay
  • Molecular techniques
  • Multiplex PCR
  • Periodontal pathogens

ASJC Scopus subject areas

  • Microbiology (medical)

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    Caposio, P., Torta, I., Romano, F., Aimetti, M., Romagnoli, R., Marchiaro, G., & Cirillo, D. M. (2003). Molecular approaches to the identification and treatment monitoring of periodontal pathogens. New Microbiologica, 26(1), 121-124.