Molecular analysis of commensal host-microbial relationships in the intestine

L. V. Hooper, Melissa Wong, A. Thelin, L. Hansson, P. G. Falk, J. I. Gordon

Research output: Contribution to journalArticle

1379 Citations (Scopus)

Abstract

Human beings contain complex societies of indigenous microbes, yet little is known about how resident bacteria shape our physiology. We colonized germ-free mice with Bacteroides thetaiotaomicron, a prominent component of the normal mouse and human intestinal microflora. Global intestinal transcriptional responses to colonization were observed with DNA microarrays, and the cellular origins of selected responses were established by laser-capture microdissection. The results reveal that this commensal bacterium modulates expression of genes involved in several important intestinal functions, including nutrient absorption, mucosal barrier fortification, xenobiotic metabolism, angiogenesis, and postnatal intestinal maturation. These findings provide perspectives about the essential nature of the interactions between resident microorganisms and their hosts.

Original languageEnglish (US)
Pages (from-to)881-884
Number of pages4
JournalScience
Volume291
Issue number5505
DOIs
StatePublished - Feb 2 2001
Externally publishedYes

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Intestines
Laser Capture Microdissection
Bacteria
Xenobiotics
Oligonucleotide Array Sequence Analysis
Gene Expression
Food
Gastrointestinal Microbiome
Bacteroides thetaiotaomicron

ASJC Scopus subject areas

  • General

Cite this

Hooper, L. V., Wong, M., Thelin, A., Hansson, L., Falk, P. G., & Gordon, J. I. (2001). Molecular analysis of commensal host-microbial relationships in the intestine. Science, 291(5505), 881-884. https://doi.org/10.1126/science.291.5505.881

Molecular analysis of commensal host-microbial relationships in the intestine. / Hooper, L. V.; Wong, Melissa; Thelin, A.; Hansson, L.; Falk, P. G.; Gordon, J. I.

In: Science, Vol. 291, No. 5505, 02.02.2001, p. 881-884.

Research output: Contribution to journalArticle

Hooper, LV, Wong, M, Thelin, A, Hansson, L, Falk, PG & Gordon, JI 2001, 'Molecular analysis of commensal host-microbial relationships in the intestine', Science, vol. 291, no. 5505, pp. 881-884. https://doi.org/10.1126/science.291.5505.881
Hooper, L. V. ; Wong, Melissa ; Thelin, A. ; Hansson, L. ; Falk, P. G. ; Gordon, J. I. / Molecular analysis of commensal host-microbial relationships in the intestine. In: Science. 2001 ; Vol. 291, No. 5505. pp. 881-884.
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