Modified toluidine blue O stain for Pneumocystis carinii: Further evaluation of some technical factors

F. G. Witebsky, J. W B Andrews, V. J. Gill, James Maclowry

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A modified toluidine blue O (TBO) stain for Pneumocystic carinii cysts was evaluated with regard to the influence of (i) the age and extent of the sulfation reagent, (ii) the source of TBO, (iii) the TBO content of the staining solution, and (iv) the amount of TBO present in the alcohol wash solutions. All TBOs evaluated, except for a new TBO obtained from Roboz Surgical Instrument Co., Inc., Washington, D.C., produced satisfactory results. Each lot of TBO should be quality controlled before use to ensure that the P. carinii cysts stain lavender against a blue background. We have ourselves decided to use only certified TBO with a high dye content. As extensively used sulfation reagent provided less satisfactory results than did either freshly prepared or 1-week-old unused sulfation reagent, we have decided to prepare fresh sulfation reagent at least weekly and to discard used sulfation reagent after 10 slides have been processed.

Original languageEnglish (US)
Pages (from-to)774-775
Number of pages2
JournalJournal of Clinical Microbiology
Volume26
Issue number4
StatePublished - 1988
Externally publishedYes

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Pneumocystis carinii
Tolonium Chloride
Coloring Agents
Cysts
Lavandula
Surgical Instruments
Alcohols
Staining and Labeling

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Modified toluidine blue O stain for Pneumocystis carinii : Further evaluation of some technical factors. / Witebsky, F. G.; Andrews, J. W B; Gill, V. J.; Maclowry, James.

In: Journal of Clinical Microbiology, Vol. 26, No. 4, 1988, p. 774-775.

Research output: Contribution to journalArticle

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