MMP-8 overexpression and persistence of neutrophils relate to stress-impaired healing and poor collagen architecture in mice

Praveen K. Gajendrareddy, Christopher G. Engeland, Roger Junges, Michael P. Horan, Isolde G. Rojas, Phillip Marucha

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinase (TIMPs) are critical for tissue remodeling during wound repair. Psychological stress has been found to impair wound healing in humans and animals. The objective of this study was to assess MMP and TIMP gene expression during stress-impaired healing. Female SKH-1 mice (n= 299) were divided into control and stress groups (13. h restraint/day for 3. days prior to and 5. days post-wounding). Two 3.5. mm cutaneous full-thickness wounds were placed on the dorsum of each mouse and wound measurements were performed daily. RT-PCR for gene expression of MMP-2, MMP-8, MMP-9, TIMP-1 and TIMP-2 was performed at days 1, 3 and 5. Immunohistochemical analyses of the healed wounds were performed at days 15 and 28. As expected, wounds healed more slowly in restraint-stressed mice compared to controls. Stressed mice exhibited MMP-8 overexpression and lower TIMP-1 levels during healing, and poorer collagen organization once healed. MMP-8 overexpression may have stemmed from a higher level of neutrophils, observed in wound tissue on days 3 and 5. These findings implicate higher neutrophil numbers, MMP-8 overexpression, and TIMP-1 under-expression, as mechanisms that may compromise wound outcomes such as scarring under conditions of stress.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalBrain, Behavior, and Immunity
Volume28
DOIs
StatePublished - Feb 2013
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 8
Neutrophils
Collagen
Wounds and Injuries
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Gene Expression
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Psychological Stress
Wound Healing
Cicatrix
Polymerase Chain Reaction
Control Groups
Skin

Keywords

  • Collagen
  • Mifepristone
  • MMP
  • Psychological stress
  • Remodeling
  • RU486
  • Scarring
  • TIMP
  • Wound healing

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

MMP-8 overexpression and persistence of neutrophils relate to stress-impaired healing and poor collagen architecture in mice. / Gajendrareddy, Praveen K.; Engeland, Christopher G.; Junges, Roger; Horan, Michael P.; Rojas, Isolde G.; Marucha, Phillip.

In: Brain, Behavior, and Immunity, Vol. 28, 02.2013, p. 44-48.

Research output: Contribution to journalArticle

Gajendrareddy, Praveen K. ; Engeland, Christopher G. ; Junges, Roger ; Horan, Michael P. ; Rojas, Isolde G. ; Marucha, Phillip. / MMP-8 overexpression and persistence of neutrophils relate to stress-impaired healing and poor collagen architecture in mice. In: Brain, Behavior, and Immunity. 2013 ; Vol. 28. pp. 44-48.
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