Mitogenic response of human thymocytes: Identification of functional Ca2+-dependent and independent signals

C. M. Roifman, G. B. Mills, R. K. Cheung, E. W. Gelfand

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    12 Scopus citations

    Abstract

    In contrast to peripheral blood mononuclear cells (PBMC), human thymocytes do not exhibit a proliferative response to the T cell mitogens phytohaemagglutinin (PHA), concanavalin A (Con A), or Staphylococcal protein A (SPA). In thymocytes and PBMC, Con A and PHA induce increases in free cytosolic calcium concentrations ([Ca2+](i)). Since both Con A and PHA induce similar increases in [Ca2+](i) in thymocytes and PBMC, the absence of thymocyte proliferation was not due to an inability to induce an increase in [Ca2+](i). The lack of a proliferative response was secondary to the failure of the mitogens to induce interleukin 2 (IL-2) production. Incubation of mitogen-treated thymocytes with phorbol esters reconstituted IL-2 production and the proliferative response indicating that the cells were indeed activated by the mitogens. Similarly, addition of exogenous recombinant IL-2 also induced mitogen-treated thymocytes to proliferate. This IL-2-dependent proliferation established that SPA, Con A, and PHA triggered the expression of biologically active IL-2 receptors. Since an increase in [Ca2+](i) is a prerequisite, and possibly a trigger, for IL-2 production, the failure of PHA, Con A, or SPA to result in thymocyte proliferation may be due to an inability of thymocytes to respond to increases in [Ca2+ ](i) with subsequent IL-2 production.

    Original languageEnglish (US)
    Pages (from-to)139-149
    Number of pages11
    JournalClinical and Experimental Immunology
    Volume66
    Issue number1
    StatePublished - Jan 1 1986

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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