Mitogenic effects of excitatory amino acids in the adult rat retina

JoséA A. Sahel; Daniel M. Albert; Simmons Lessell; Henry Adler; Terri L. McGee; Joan Konrad-Rastegar

doi:10.1016/0014-4835(91)90226-5

Mitogenic effects of excitatory amino acids in the adult rat retina

JoséA A. Sahel, Daniel M. Albert, Simmons Lessell, Henry Adler, Terri L. McGee, Joan Konrad-Rastegar

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We studied the retinas of adult rats after the intravitreal injection of excitatory amino acids and ouabain. Kainic acid, domoic acid, N-methyl d-asparate and ouabain produced swelling and vacuolization of the outer plexiform, inner nuclear and inner plexiform layers and pyknosis. Mitoses were present in retinas treated with all agents other than N-methyl d-asparate. Rompun ketamine anesthesia blocked the mitogenic effects. Immunohistochemical labeling of both glial fibrillary acidic protein and S100 protein would indicate that the mitoses are occurring in glial cells. We suggest that the mitogenic effects are mediated through action on glial cationic channels, and might account for the reactive gliosis observed in some retinal lesions.

Original language	English (US)
Pages (from-to)	657-664
Number of pages	8
Journal	Experimental Eye Research
Volume	53
Issue number	5
DOIs	https://doi.org/10.1016/0014-4835(91)90226-5
State	Published - Nov 1991
Externally published	Yes

Keywords

S100 protein
excitatory amino acids
glia
glial fibrillary acidic protein
kainic acid
ouabain

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/0014-4835(91)90226-5

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title = "Mitogenic effects of excitatory amino acids in the adult rat retina",

abstract = "We studied the retinas of adult rats after the intravitreal injection of excitatory amino acids and ouabain. Kainic acid, domoic acid, N-methyl d-asparate and ouabain produced swelling and vacuolization of the outer plexiform, inner nuclear and inner plexiform layers and pyknosis. Mitoses were present in retinas treated with all agents other than N-methyl d-asparate. Rompun ketamine anesthesia blocked the mitogenic effects. Immunohistochemical labeling of both glial fibrillary acidic protein and S100 protein would indicate that the mitoses are occurring in glial cells. We suggest that the mitogenic effects are mediated through action on glial cationic channels, and might account for the reactive gliosis observed in some retinal lesions.",

keywords = "S100 protein, excitatory amino acids, glia, glial fibrillary acidic protein, kainic acid, ouabain",

author = "Sahel, {Jos{\'e}A A.} and Albert, {Daniel M.} and Simmons Lessell and Henry Adler and McGee, {Terri L.} and Joan Konrad-Rastegar",

note = "Funding Information: The authors wish to acknowledge John E. Dowling, Ph.D., for his valuable help and his suggestions: Don Pure, M.D.. Ph.D. for valuable discussions from in vitro studies; Susan Edgerton and Ann Thor. M.D. for their expert help in immunocytochemistry. and Charles Specht, M.D. for his work at the early stages of this study. This work was supported in part by grants from NE1 EY01917 (D.M.A.), Fondation pour la Recherche Therapeutique, Paris 0. A. S.) and Research to Prevent Blindness, New York (].A. S.-Research to Prevent Blindness International Research Scholar).",

year = "1991",

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language = "English (US)",

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AU - Albert, Daniel M.

AU - Lessell, Simmons

AU - Adler, Henry

AU - McGee, Terri L.

AU - Konrad-Rastegar, Joan

N1 - Funding Information: The authors wish to acknowledge John E. Dowling, Ph.D., for his valuable help and his suggestions: Don Pure, M.D.. Ph.D. for valuable discussions from in vitro studies; Susan Edgerton and Ann Thor. M.D. for their expert help in immunocytochemistry. and Charles Specht, M.D. for his work at the early stages of this study. This work was supported in part by grants from NE1 EY01917 (D.M.A.), Fondation pour la Recherche Therapeutique, Paris 0. A. S.) and Research to Prevent Blindness, New York (].A. S.-Research to Prevent Blindness International Research Scholar).

PY - 1991/11

Y1 - 1991/11

N2 - We studied the retinas of adult rats after the intravitreal injection of excitatory amino acids and ouabain. Kainic acid, domoic acid, N-methyl d-asparate and ouabain produced swelling and vacuolization of the outer plexiform, inner nuclear and inner plexiform layers and pyknosis. Mitoses were present in retinas treated with all agents other than N-methyl d-asparate. Rompun ketamine anesthesia blocked the mitogenic effects. Immunohistochemical labeling of both glial fibrillary acidic protein and S100 protein would indicate that the mitoses are occurring in glial cells. We suggest that the mitogenic effects are mediated through action on glial cationic channels, and might account for the reactive gliosis observed in some retinal lesions.

AB - We studied the retinas of adult rats after the intravitreal injection of excitatory amino acids and ouabain. Kainic acid, domoic acid, N-methyl d-asparate and ouabain produced swelling and vacuolization of the outer plexiform, inner nuclear and inner plexiform layers and pyknosis. Mitoses were present in retinas treated with all agents other than N-methyl d-asparate. Rompun ketamine anesthesia blocked the mitogenic effects. Immunohistochemical labeling of both glial fibrillary acidic protein and S100 protein would indicate that the mitoses are occurring in glial cells. We suggest that the mitogenic effects are mediated through action on glial cationic channels, and might account for the reactive gliosis observed in some retinal lesions.

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KW - glial fibrillary acidic protein

KW - kainic acid

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Mitogenic effects of excitatory amino acids in the adult rat retina

Abstract

Keywords

ASJC Scopus subject areas

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