Mitogenic effects of excitatory amino acids in the adult rat retina

JoséA A. Sahel, Daniel Albert, Simmons Lessell, Henry Adler, Terri L. McGee, Joan Konrad-Rastegar

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We studied the retinas of adult rats after the intravitreal injection of excitatory amino acids and ouabain. Kainic acid, domoic acid, N-methyl d-asparate and ouabain produced swelling and vacuolization of the outer plexiform, inner nuclear and inner plexiform layers and pyknosis. Mitoses were present in retinas treated with all agents other than N-methyl d-asparate. Rompun ketamine anesthesia blocked the mitogenic effects. Immunohistochemical labeling of both glial fibrillary acidic protein and S100 protein would indicate that the mitoses are occurring in glial cells. We suggest that the mitogenic effects are mediated through action on glial cationic channels, and might account for the reactive gliosis observed in some retinal lesions.

Original languageEnglish (US)
Pages (from-to)657-664
Number of pages8
JournalExperimental Eye Research
Volume53
Issue number5
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Excitatory Amino Acids
Ouabain
Mitosis
Neuroglia
Retina
Xylazine
Intravitreal Injections
Gliosis
S100 Proteins
Kainic Acid
Glial Fibrillary Acidic Protein
Ketamine
Anesthesia
domoic acid

Keywords

  • excitatory amino acids
  • glia
  • glial fibrillary acidic protein
  • kainic acid
  • ouabain
  • S100 protein

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Sahel, J. A., Albert, D., Lessell, S., Adler, H., McGee, T. L., & Konrad-Rastegar, J. (1991). Mitogenic effects of excitatory amino acids in the adult rat retina. Experimental Eye Research, 53(5), 657-664. https://doi.org/10.1016/0014-4835(91)90226-5

Mitogenic effects of excitatory amino acids in the adult rat retina. / Sahel, JoséA A.; Albert, Daniel; Lessell, Simmons; Adler, Henry; McGee, Terri L.; Konrad-Rastegar, Joan.

In: Experimental Eye Research, Vol. 53, No. 5, 01.01.1991, p. 657-664.

Research output: Contribution to journalArticle

Sahel, JA, Albert, D, Lessell, S, Adler, H, McGee, TL & Konrad-Rastegar, J 1991, 'Mitogenic effects of excitatory amino acids in the adult rat retina', Experimental Eye Research, vol. 53, no. 5, pp. 657-664. https://doi.org/10.1016/0014-4835(91)90226-5
Sahel, JoséA A. ; Albert, Daniel ; Lessell, Simmons ; Adler, Henry ; McGee, Terri L. ; Konrad-Rastegar, Joan. / Mitogenic effects of excitatory amino acids in the adult rat retina. In: Experimental Eye Research. 1991 ; Vol. 53, No. 5. pp. 657-664.
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