Mitochondrial p32 is upregulated in Myc expressing brain cancers and mediates glutamine addiction

Valentina Fogal, Ivan Babic, Ying Chao, Sandra Pastorino, Rajesh Mukthavaram, Pengfei Jiang, Yoon-Jae Cho, Sandeep C. Pingle, John R. Crawford, David E. Piccioni, Santosh Kesari

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Metabolic reprogramming is a key feature of tumorigenesis that is controlled by oncogenes. Enhanced utilization of glucose and glutamine are the best-established hallmarks of tumor metabolism. The oncogene c-Myc is one of the major players responsible for this metabolic alteration. However, the molecular mechanisms involved in Myc-induced metabolic reprogramming are not well defined. Here we identify p32, a mitochondrial protein known to play a role in the expression of mitochondrial respiratory chain complexes, as a critical player in Myc-induced glutamine addiction. We show that p32 is a direct transcriptional target of Myc and that high level of Myc in malignant brain cancers correlates with high expression of p32. Attenuation of p32 expression reduced growth rate of glioma cells expressing Myc and impaired tumor formation in vivo. Loss of p32 in glutamine addicted glioma cells induced resistance to glutamine deprivation and imparted sensitivity to glucose withdrawal. Finally, we provide evidence that p32 expression contributes to Myc-induced glutamine addiction of cancer cells. Our findings suggest that Myc promotes the expression of p32, which is required to maintain sufficient respiratory capacity to sustain glutamine metabolism in Myc transformed cells.

Original languageEnglish (US)
Pages (from-to)1157-1170
Number of pages14
JournalOncotarget
Volume6
Issue number2
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Glutamine
Brain Neoplasms
Glioma
Glucose
Neoplasms
myc Genes
Mitochondrial Proteins
Electron Transport
Oncogenes
Carcinogenesis
Growth

Keywords

  • Brain cancer
  • C-Myc
  • Glutamine addiction
  • Mitochondrial p32

ASJC Scopus subject areas

  • Oncology

Cite this

Fogal, V., Babic, I., Chao, Y., Pastorino, S., Mukthavaram, R., Jiang, P., ... Kesari, S. (2015). Mitochondrial p32 is upregulated in Myc expressing brain cancers and mediates glutamine addiction. Oncotarget, 6(2), 1157-1170. https://doi.org/10.18632/oncotarget.2708

Mitochondrial p32 is upregulated in Myc expressing brain cancers and mediates glutamine addiction. / Fogal, Valentina; Babic, Ivan; Chao, Ying; Pastorino, Sandra; Mukthavaram, Rajesh; Jiang, Pengfei; Cho, Yoon-Jae; Pingle, Sandeep C.; Crawford, John R.; Piccioni, David E.; Kesari, Santosh.

In: Oncotarget, Vol. 6, No. 2, 01.01.2015, p. 1157-1170.

Research output: Contribution to journalArticle

Fogal, V, Babic, I, Chao, Y, Pastorino, S, Mukthavaram, R, Jiang, P, Cho, Y-J, Pingle, SC, Crawford, JR, Piccioni, DE & Kesari, S 2015, 'Mitochondrial p32 is upregulated in Myc expressing brain cancers and mediates glutamine addiction', Oncotarget, vol. 6, no. 2, pp. 1157-1170. https://doi.org/10.18632/oncotarget.2708
Fogal V, Babic I, Chao Y, Pastorino S, Mukthavaram R, Jiang P et al. Mitochondrial p32 is upregulated in Myc expressing brain cancers and mediates glutamine addiction. Oncotarget. 2015 Jan 1;6(2):1157-1170. https://doi.org/10.18632/oncotarget.2708
Fogal, Valentina ; Babic, Ivan ; Chao, Ying ; Pastorino, Sandra ; Mukthavaram, Rajesh ; Jiang, Pengfei ; Cho, Yoon-Jae ; Pingle, Sandeep C. ; Crawford, John R. ; Piccioni, David E. ; Kesari, Santosh. / Mitochondrial p32 is upregulated in Myc expressing brain cancers and mediates glutamine addiction. In: Oncotarget. 2015 ; Vol. 6, No. 2. pp. 1157-1170.
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