MiR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR

Mumtaz Y. Balkhi, O. Hans Iwenofu, Nadine Bakkar, Katherine J. Ladner, Dawn S. Chandler, Peter J. Houghton, Cheryl A. London, William Kraybill, Danilo Perrotti, Carlo M. Croce, Charles Keller, Denis C. Guttridge

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Abstract

In sarcoma, the activity of NF-κB (nuclear factor κB) reduces the abundance of the microRNA (miRNA) miR-29. The tumor suppressor A20 [also known as TNFAIP3 (tumor necrosis factor-α-induced protein 3)] inhibits an upstream activator of NF-kB and is often mutated in lymphomas. In a panel of human sarcoma cell lines, we found that the activation of NF-κB was increased and, although the abundance of A20 protein and mRNA was decreased, the gene encoding A20 was rarely mutated. The 3′ untranslated region (UTR) of A20 mRNA has conserved binding sites for both of the miRNAs miR-29 and miR-125. Whereas the expression of miR-125 was increased in human sarcoma tissue, that of miR-29 was decreased in most samples. Overexpression of miR-125 decreased the abundance of A20 mRNA, whereas reconstituting miR-29 in sarcoma cell lines increased the abundance of A20 mRNA and protein. By interacting directly with the RNA binding protein HuR (human antigen R; also known as ELAVL1), miR-29 prevented HuR from binding to the A20 3′UTR and recruiting the RNA degradation complex RISC (RNA-induced silencing complex), suggesting that miR-29 can act as a decoy for HuR, thus protecting A20 transcripts. Decreased miR-29 and A20 abundance in sarcomas correlated with increased activity of NF-kB and decreased expression of genes associated with differentiation. Together, the findings reveal a unique role of miR-29 and suggest that its absence may contribute to sarcoma tumorigenesis.

Original languageEnglish (US)
JournalScience Signaling
Volume6
Issue number286
DOIs
StatePublished - Jul 30 2013

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RNA Stability
Sarcoma
Tumors
Degradation
Messenger RNA
NF-kappa B
MicroRNAs
Neoplasms
RNA-Induced Silencing Complex
Cells
Proteins
Gene encoding
RNA-Binding Proteins
3' Untranslated Regions
Cell Line
Tumor Necrosis Factor-alpha
Genes
Chemical activation
Binding Sites
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Balkhi, M. Y., Iwenofu, O. H., Bakkar, N., Ladner, K. J., Chandler, D. S., Houghton, P. J., ... Guttridge, D. C. (2013). MiR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR. Science Signaling, 6(286). https://doi.org/10.1126/scisignal.2004177

MiR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR. / Balkhi, Mumtaz Y.; Iwenofu, O. Hans; Bakkar, Nadine; Ladner, Katherine J.; Chandler, Dawn S.; Houghton, Peter J.; London, Cheryl A.; Kraybill, William; Perrotti, Danilo; Croce, Carlo M.; Keller, Charles; Guttridge, Denis C.

In: Science Signaling, Vol. 6, No. 286, 30.07.2013.

Research output: Contribution to journalArticle

Balkhi, MY, Iwenofu, OH, Bakkar, N, Ladner, KJ, Chandler, DS, Houghton, PJ, London, CA, Kraybill, W, Perrotti, D, Croce, CM, Keller, C & Guttridge, DC 2013, 'MiR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR', Science Signaling, vol. 6, no. 286. https://doi.org/10.1126/scisignal.2004177
Balkhi, Mumtaz Y. ; Iwenofu, O. Hans ; Bakkar, Nadine ; Ladner, Katherine J. ; Chandler, Dawn S. ; Houghton, Peter J. ; London, Cheryl A. ; Kraybill, William ; Perrotti, Danilo ; Croce, Carlo M. ; Keller, Charles ; Guttridge, Denis C. / MiR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR. In: Science Signaling. 2013 ; Vol. 6, No. 286.
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AU - Ladner, Katherine J.

AU - Chandler, Dawn S.

AU - Houghton, Peter J.

AU - London, Cheryl A.

AU - Kraybill, William

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AU - Croce, Carlo M.

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