MicroRNA signatures of colonic polyps on screening and histology

Vassiliki Tsikitis, Amiee Potter, Motomi (Tomi) Mori, Julie A. Buckmeier, Christina R. Preece, Christina (Chris) Harrington, Angela N. Bartley, Achyut K. Bhattacharyya, Stanley R. Hamilton, M. Peter Lance, Patricia A. Thompson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Colorectal cancer and adenoma adjacent to cancer exhibit distinct microRNA (miRNA) alterations in an apparent mucosato-adenocarcinoma sequence. The pattern of microRNAs in screen-detected polyps in relation to histologic features and cancer risk has not been investigated. miRNA expression analysis was performed on normal mucosa (NM), hyperplastic polyps (HP), tubular adenomas (TA), tubulovillous adenomas or high-grade dysplasia (TVHG), and serrated polyps [sessile serrated adenoma/polyps (SSA/P) and traditional serrated adenomas (TSA)] in biopsy specimens from 109 patients undergoing screening/surveillance colonoscopy. Generalized linear models were used to identify differentially expressed miRNAs by histologic type and logistic regression to identify miRNA predictors of histopathology. False discovery rate (FDR) was used to control for multiple comparisons. We identified 99 miRNAs differing in at least one of five histopathologic groups (FDR ≤0.05). In a comparison of HPNM versus TVHG, the top most upregulated and downregulated miRNAs in HPNM included miR-145,-143,-107,-194, and-26a (upregulated), and miR-663,-1268,-320b,-1275, and-320b (downregulated; FDR P 0.05). miR-145 and-619 showed high accuracy to discriminate low-from highrisk polyps without serrated histology (TVHG vs. HPNM \+ TA; CI, 95.6%), whereas miR-124,-143, and-30a showed high accuracy of separating high-risk polyps (TVHG \+ TSA) from low-risk polyps (HPNM \+ TA \+ SSA/P; CI, 96.0%). For TSAs, miR-125b and-199a were uniquely downregulated relative to HPNMs, and miR-335,-222, and-214 discriminated between non-serrated and serrated histology. Our data support the presence of colorectal cancer-associated miRNA alterations in screen-detected adenomas that may be useful for risk stratification for surveillance interval planning.

Original languageEnglish (US)
Pages (from-to)942-949
Number of pages8
JournalCancer Prevention Research
Volume9
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Colonic Polyps
MicroRNAs
Adenoma
Histology
Polyps
Down-Regulation
Colorectal Neoplasms
Colonoscopy
Linear Models
Neoplasms
Mucous Membrane
Adenocarcinoma
Logistic Models

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

MicroRNA signatures of colonic polyps on screening and histology. / Tsikitis, Vassiliki; Potter, Amiee; Mori, Motomi (Tomi); Buckmeier, Julie A.; Preece, Christina R.; Harrington, Christina (Chris); Bartley, Angela N.; Bhattacharyya, Achyut K.; Hamilton, Stanley R.; Lance, M. Peter; Thompson, Patricia A.

In: Cancer Prevention Research, Vol. 9, No. 12, 01.12.2016, p. 942-949.

Research output: Contribution to journalArticle

Tsikitis, V, Potter, A, Mori, MT, Buckmeier, JA, Preece, CR, Harrington, CC, Bartley, AN, Bhattacharyya, AK, Hamilton, SR, Lance, MP & Thompson, PA 2016, 'MicroRNA signatures of colonic polyps on screening and histology', Cancer Prevention Research, vol. 9, no. 12, pp. 942-949. https://doi.org/10.1158/1940-6207.CAPR-16-0086
Tsikitis, Vassiliki ; Potter, Amiee ; Mori, Motomi (Tomi) ; Buckmeier, Julie A. ; Preece, Christina R. ; Harrington, Christina (Chris) ; Bartley, Angela N. ; Bhattacharyya, Achyut K. ; Hamilton, Stanley R. ; Lance, M. Peter ; Thompson, Patricia A. / MicroRNA signatures of colonic polyps on screening and histology. In: Cancer Prevention Research. 2016 ; Vol. 9, No. 12. pp. 942-949.
@article{100d6c5f470d4cceb0905626b0a9adcd,
title = "MicroRNA signatures of colonic polyps on screening and histology",
abstract = "Colorectal cancer and adenoma adjacent to cancer exhibit distinct microRNA (miRNA) alterations in an apparent mucosato-adenocarcinoma sequence. The pattern of microRNAs in screen-detected polyps in relation to histologic features and cancer risk has not been investigated. miRNA expression analysis was performed on normal mucosa (NM), hyperplastic polyps (HP), tubular adenomas (TA), tubulovillous adenomas or high-grade dysplasia (TVHG), and serrated polyps [sessile serrated adenoma/polyps (SSA/P) and traditional serrated adenomas (TSA)] in biopsy specimens from 109 patients undergoing screening/surveillance colonoscopy. Generalized linear models were used to identify differentially expressed miRNAs by histologic type and logistic regression to identify miRNA predictors of histopathology. False discovery rate (FDR) was used to control for multiple comparisons. We identified 99 miRNAs differing in at least one of five histopathologic groups (FDR ≤0.05). In a comparison of HPNM versus TVHG, the top most upregulated and downregulated miRNAs in HPNM included miR-145,-143,-107,-194, and-26a (upregulated), and miR-663,-1268,-320b,-1275, and-320b (downregulated; FDR P 0.05). miR-145 and-619 showed high accuracy to discriminate low-from highrisk polyps without serrated histology (TVHG vs. HPNM \+ TA; CI, 95.6{\%}), whereas miR-124,-143, and-30a showed high accuracy of separating high-risk polyps (TVHG \+ TSA) from low-risk polyps (HPNM \+ TA \+ SSA/P; CI, 96.0{\%}). For TSAs, miR-125b and-199a were uniquely downregulated relative to HPNMs, and miR-335,-222, and-214 discriminated between non-serrated and serrated histology. Our data support the presence of colorectal cancer-associated miRNA alterations in screen-detected adenomas that may be useful for risk stratification for surveillance interval planning.",
author = "Vassiliki Tsikitis and Amiee Potter and Mori, {Motomi (Tomi)} and Buckmeier, {Julie A.} and Preece, {Christina R.} and Harrington, {Christina (Chris)} and Bartley, {Angela N.} and Bhattacharyya, {Achyut K.} and Hamilton, {Stanley R.} and Lance, {M. Peter} and Thompson, {Patricia A.}",
year = "2016",
month = "12",
day = "1",
doi = "10.1158/1940-6207.CAPR-16-0086",
language = "English (US)",
volume = "9",
pages = "942--949",
journal = "Cancer Prevention Research",
issn = "1940-6207",
publisher = "American Association for Cancer Research Inc.",
number = "12",

}

TY - JOUR

T1 - MicroRNA signatures of colonic polyps on screening and histology

AU - Tsikitis, Vassiliki

AU - Potter, Amiee

AU - Mori, Motomi (Tomi)

AU - Buckmeier, Julie A.

AU - Preece, Christina R.

AU - Harrington, Christina (Chris)

AU - Bartley, Angela N.

AU - Bhattacharyya, Achyut K.

AU - Hamilton, Stanley R.

AU - Lance, M. Peter

AU - Thompson, Patricia A.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Colorectal cancer and adenoma adjacent to cancer exhibit distinct microRNA (miRNA) alterations in an apparent mucosato-adenocarcinoma sequence. The pattern of microRNAs in screen-detected polyps in relation to histologic features and cancer risk has not been investigated. miRNA expression analysis was performed on normal mucosa (NM), hyperplastic polyps (HP), tubular adenomas (TA), tubulovillous adenomas or high-grade dysplasia (TVHG), and serrated polyps [sessile serrated adenoma/polyps (SSA/P) and traditional serrated adenomas (TSA)] in biopsy specimens from 109 patients undergoing screening/surveillance colonoscopy. Generalized linear models were used to identify differentially expressed miRNAs by histologic type and logistic regression to identify miRNA predictors of histopathology. False discovery rate (FDR) was used to control for multiple comparisons. We identified 99 miRNAs differing in at least one of five histopathologic groups (FDR ≤0.05). In a comparison of HPNM versus TVHG, the top most upregulated and downregulated miRNAs in HPNM included miR-145,-143,-107,-194, and-26a (upregulated), and miR-663,-1268,-320b,-1275, and-320b (downregulated; FDR P 0.05). miR-145 and-619 showed high accuracy to discriminate low-from highrisk polyps without serrated histology (TVHG vs. HPNM \+ TA; CI, 95.6%), whereas miR-124,-143, and-30a showed high accuracy of separating high-risk polyps (TVHG \+ TSA) from low-risk polyps (HPNM \+ TA \+ SSA/P; CI, 96.0%). For TSAs, miR-125b and-199a were uniquely downregulated relative to HPNMs, and miR-335,-222, and-214 discriminated between non-serrated and serrated histology. Our data support the presence of colorectal cancer-associated miRNA alterations in screen-detected adenomas that may be useful for risk stratification for surveillance interval planning.

AB - Colorectal cancer and adenoma adjacent to cancer exhibit distinct microRNA (miRNA) alterations in an apparent mucosato-adenocarcinoma sequence. The pattern of microRNAs in screen-detected polyps in relation to histologic features and cancer risk has not been investigated. miRNA expression analysis was performed on normal mucosa (NM), hyperplastic polyps (HP), tubular adenomas (TA), tubulovillous adenomas or high-grade dysplasia (TVHG), and serrated polyps [sessile serrated adenoma/polyps (SSA/P) and traditional serrated adenomas (TSA)] in biopsy specimens from 109 patients undergoing screening/surveillance colonoscopy. Generalized linear models were used to identify differentially expressed miRNAs by histologic type and logistic regression to identify miRNA predictors of histopathology. False discovery rate (FDR) was used to control for multiple comparisons. We identified 99 miRNAs differing in at least one of five histopathologic groups (FDR ≤0.05). In a comparison of HPNM versus TVHG, the top most upregulated and downregulated miRNAs in HPNM included miR-145,-143,-107,-194, and-26a (upregulated), and miR-663,-1268,-320b,-1275, and-320b (downregulated; FDR P 0.05). miR-145 and-619 showed high accuracy to discriminate low-from highrisk polyps without serrated histology (TVHG vs. HPNM \+ TA; CI, 95.6%), whereas miR-124,-143, and-30a showed high accuracy of separating high-risk polyps (TVHG \+ TSA) from low-risk polyps (HPNM \+ TA \+ SSA/P; CI, 96.0%). For TSAs, miR-125b and-199a were uniquely downregulated relative to HPNMs, and miR-335,-222, and-214 discriminated between non-serrated and serrated histology. Our data support the presence of colorectal cancer-associated miRNA alterations in screen-detected adenomas that may be useful for risk stratification for surveillance interval planning.

UR - http://www.scopus.com/inward/record.url?scp=85008323066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008323066&partnerID=8YFLogxK

U2 - 10.1158/1940-6207.CAPR-16-0086

DO - 10.1158/1940-6207.CAPR-16-0086

M3 - Article

VL - 9

SP - 942

EP - 949

JO - Cancer Prevention Research

JF - Cancer Prevention Research

SN - 1940-6207

IS - 12

ER -