Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells

Stefanie Giera, Rong Luo, Yanqin Ying, Sarah D. Ackerman, Sung Jin Jeong, Hannah M. Stoveken, Christopher J. Folts, Christina A. Welsh, Gregory G. Tall, Beth Stevens, Kelly Monk, Xianhua Piao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the central nervous system (CNS), myelin formation and repair are regulated by oligodendrocyte (OL) lineage cells, which sense and integrate signals from their environment, including from other glial cells and the extracellular matrix (ECM). The signaling pathways that coordinate this complex communication, however, remain poorly understood. The adhesion G protein-coupled receptor ADGRG1 (also known as GPR56) is an evolutionarily conserved regulator of OL development in humans, mice, and zebrafish, although its activating ligand for OL lineage cells is unknown. Here, we report that microglia-derived transglutaminase-2 (TG2) signals to ADGRG1 on OL precursor cells (OPCs) in the presence of the ECM protein laminin and that TG2/ laminin-dependent activation of ADGRG1 promotes OPC proliferation. Signaling by TG2/laminin to ADGRG1 on OPCs additionally improves remyelination in two murine models of demyelination. These findings identify a novel glia-to-glia signaling pathway that promotes myelin formation and repair, and suggest new strategies to enhance remyelination.

Original languageEnglish (US)
Article numbere33385
JournaleLife
Volume7
DOIs
StatePublished - May 29 2018

Fingerprint

Oligodendroglia
Laminin
Myelin Sheath
Repair
Neuroglia
Extracellular Matrix Proteins
Cell proliferation
Neurology
G-Protein-Coupled Receptors
Microglia
Human Development
Demyelinating Diseases
Zebrafish
Adhesion
Chemical activation
Ligands
Extracellular Matrix
Central Nervous System
Communication
Cell Proliferation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Giera, S., Luo, R., Ying, Y., Ackerman, S. D., Jeong, S. J., Stoveken, H. M., ... Piao, X. (2018). Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells. eLife, 7, [e33385]. https://doi.org/10.7554/eLife.33385

Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells. / Giera, Stefanie; Luo, Rong; Ying, Yanqin; Ackerman, Sarah D.; Jeong, Sung Jin; Stoveken, Hannah M.; Folts, Christopher J.; Welsh, Christina A.; Tall, Gregory G.; Stevens, Beth; Monk, Kelly; Piao, Xianhua.

In: eLife, Vol. 7, e33385, 29.05.2018.

Research output: Contribution to journalArticle

Giera, S, Luo, R, Ying, Y, Ackerman, SD, Jeong, SJ, Stoveken, HM, Folts, CJ, Welsh, CA, Tall, GG, Stevens, B, Monk, K & Piao, X 2018, 'Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells', eLife, vol. 7, e33385. https://doi.org/10.7554/eLife.33385
Giera, Stefanie ; Luo, Rong ; Ying, Yanqin ; Ackerman, Sarah D. ; Jeong, Sung Jin ; Stoveken, Hannah M. ; Folts, Christopher J. ; Welsh, Christina A. ; Tall, Gregory G. ; Stevens, Beth ; Monk, Kelly ; Piao, Xianhua. / Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells. In: eLife. 2018 ; Vol. 7.
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