@inproceedings{a155aacdff2b42ada913132e47d48223,
title = "Microfluidic vortex technology for pure circulating tumor cell concentration from patient blood",
abstract = "The isolation of circulating tumor cells (CTCs) from blood is important for determining cancer prognosis, characterizing genetic mutations for targeted drug therapies, and studying the biological mechanisms of metastasis. Here, we combine microfluidic inertial focusing and vortices to extract and enrich large CTCs from a significant background of smaller leukocytes. The size-based, clog-less technique operates at a high throughput (20 min for 7.5 mL of blood) and delivers a concentrated sample (300 μL volume) of cells at high purity (57-94%) and viability (85.7%). We demonstrate successful CTC extraction from stage IV cancer patients with breast (N=4) and lung (N=8) cancer.",
keywords = "Cancer cell trapping, Circulating tumor cells, Inertial microfluidics, Rare cell enrichment, Vortex",
author = "J. Che and E. Sollier and Go, {D. E.} and N. Kummer and M. Rettig and J. Goldman and N. Nickols and S. McCloskey and Kulkarni, {R. P.} and {Di Carlo}, Dino",
year = "2013",
language = "English (US)",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1018--1020",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}