Mice deficient in Six5 develop cataracts: Implications for myotonic dystrophy

Todd R. Klesert, Diane H. Cho, John I. Clark, James Maylie, John Adelman, Lauren Snider, Eric C. Yuen, Philippe Soriano, Stephen J. Tapscott

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Expansion of a CTG trinucleotide repeat in the 3' UTR of the gene DMPK at the DM1 locus on chromosome 19 causes myotonic dystrophy a dominantly inherited disease characterized by skeletal muscle dystrophy and myotonia, cataracts and cardiac conduction defects. Targeted deletion of Dm15, the mouse orthologue of human DMPK, produced mice with a mild myopathy and cardiac conduction abnormalities, but without other features of myotonic dystrophy, such as myotonia and cataracts. We, and others, have demonstrated that repeat expansion decreases expression of the adjacent gene SIX5 (refs 7,8), which encodes a home-odomain transcription factor. To determine whether SIX5 deficiency contributes to the myotonic dystrophy phenotype, we disrupted mouse Six5 by replacing the first exon with a β-galactosidase reporter. Six5-mutant mice showed reporter expression in multiple tissues, including the developing lens. Homozygous mutant mice had no apparent abnormalities of skeletal muscle function, but developed lenticular opacities at a higher rate than controls. Our results suggest that SIX5 deficiency contributes to the cataract phenotype in myotonic dystrophy, and that myotonic dystrophy represents a multigenic disorder.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalNature Genetics
Volume25
Issue number1
DOIs
StatePublished - May 2000

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Myotonic Dystrophy
Cataract
Myotonia
Skeletal Muscle
Galactosidases
Phenotype
Chromosomes, Human, Pair 19
Trinucleotide Repeats
3' Untranslated Regions
Muscular Diseases
Lenses
Exons
Transcription Factors
Gene Expression
Genes

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Klesert, T. R., Cho, D. H., Clark, J. I., Maylie, J., Adelman, J., Snider, L., ... Tapscott, S. J. (2000). Mice deficient in Six5 develop cataracts: Implications for myotonic dystrophy. Nature Genetics, 25(1), 105-109. https://doi.org/10.1038/75490

Mice deficient in Six5 develop cataracts : Implications for myotonic dystrophy. / Klesert, Todd R.; Cho, Diane H.; Clark, John I.; Maylie, James; Adelman, John; Snider, Lauren; Yuen, Eric C.; Soriano, Philippe; Tapscott, Stephen J.

In: Nature Genetics, Vol. 25, No. 1, 05.2000, p. 105-109.

Research output: Contribution to journalArticle

Klesert, TR, Cho, DH, Clark, JI, Maylie, J, Adelman, J, Snider, L, Yuen, EC, Soriano, P & Tapscott, SJ 2000, 'Mice deficient in Six5 develop cataracts: Implications for myotonic dystrophy', Nature Genetics, vol. 25, no. 1, pp. 105-109. https://doi.org/10.1038/75490
Klesert, Todd R. ; Cho, Diane H. ; Clark, John I. ; Maylie, James ; Adelman, John ; Snider, Lauren ; Yuen, Eric C. ; Soriano, Philippe ; Tapscott, Stephen J. / Mice deficient in Six5 develop cataracts : Implications for myotonic dystrophy. In: Nature Genetics. 2000 ; Vol. 25, No. 1. pp. 105-109.
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