Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials

Kathryn McKenna, Monica Hinds, Rebecca C. Sarao, Ping Cheng Wu, Cheryl Maslen, Robert W. Glanville, Darcie Babcock, Kenton Gregory

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and cell compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36 ± 0.05 MPa and elastic moduli of 0.15 ± 0.04 and 0.91 ± 0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mm Hg were obtained from 4 mm internal diameter scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial cell growth with typical endothelial cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular cell compatibility.

Original languageEnglish (US)
Pages (from-to)225-233
Number of pages9
JournalActa Biomaterialia
Volume8
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Tropoelastin
Biocompatible Materials
Biomaterials
Grafts
Blood Vessels
Endothelial cells
Transplants
Scaffolds
Mechanical properties
Elastin
Tensile testing
Cell growth
Cell culture
Structural properties
Tensile strength
Elastic moduli
Tissue
Endothelial Cells
Fibers
Tunica Intima

Keywords

  • Electrospinning
  • Mechanical properties
  • Tissue engineering
  • Tropoelastin
  • Vascular grafts

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials. / McKenna, Kathryn; Hinds, Monica; Sarao, Rebecca C.; Wu, Ping Cheng; Maslen, Cheryl; Glanville, Robert W.; Babcock, Darcie; Gregory, Kenton.

In: Acta Biomaterialia, Vol. 8, No. 1, 01.2012, p. 225-233.

Research output: Contribution to journalArticle

McKenna, Kathryn ; Hinds, Monica ; Sarao, Rebecca C. ; Wu, Ping Cheng ; Maslen, Cheryl ; Glanville, Robert W. ; Babcock, Darcie ; Gregory, Kenton. / Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials. In: Acta Biomaterialia. 2012 ; Vol. 8, No. 1. pp. 225-233.
@article{4ed10667cc4f4169bee1cdcc5b6bf4f3,
title = "Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials",
abstract = "The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and cell compatibility evaluations from 15 wt.{\%} rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36 ± 0.05 MPa and elastic moduli of 0.15 ± 0.04 and 0.91 ± 0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mm Hg were obtained from 4 mm internal diameter scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial cell growth with typical endothelial cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular cell compatibility.",
keywords = "Electrospinning, Mechanical properties, Tissue engineering, Tropoelastin, Vascular grafts",
author = "Kathryn McKenna and Monica Hinds and Sarao, {Rebecca C.} and Wu, {Ping Cheng} and Cheryl Maslen and Glanville, {Robert W.} and Darcie Babcock and Kenton Gregory",
year = "2012",
month = "1",
doi = "10.1016/j.actbio.2011.08.001",
language = "English (US)",
volume = "8",
pages = "225--233",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials

AU - McKenna, Kathryn

AU - Hinds, Monica

AU - Sarao, Rebecca C.

AU - Wu, Ping Cheng

AU - Maslen, Cheryl

AU - Glanville, Robert W.

AU - Babcock, Darcie

AU - Gregory, Kenton

PY - 2012/1

Y1 - 2012/1

N2 - The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and cell compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36 ± 0.05 MPa and elastic moduli of 0.15 ± 0.04 and 0.91 ± 0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mm Hg were obtained from 4 mm internal diameter scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial cell growth with typical endothelial cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular cell compatibility.

AB - The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and cell compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36 ± 0.05 MPa and elastic moduli of 0.15 ± 0.04 and 0.91 ± 0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mm Hg were obtained from 4 mm internal diameter scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial cell growth with typical endothelial cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular cell compatibility.

KW - Electrospinning

KW - Mechanical properties

KW - Tissue engineering

KW - Tropoelastin

KW - Vascular grafts

UR - http://www.scopus.com/inward/record.url?scp=84855972585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855972585&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2011.08.001

DO - 10.1016/j.actbio.2011.08.001

M3 - Article

VL - 8

SP - 225

EP - 233

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 1

ER -