Measuring carboxypeptidase A activity with a centrifugal analyzer

Analytical and clinical considerations

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This adaptation of a commercially available kit for automated measurement of carboxypeptidase A (CPA; EC 3.4.17.1) activity in serum with the Cobas Bio centrifugal analyzer extends the linear range to an activity concentration of 82 U/L. Results obtained by the described method correlated closely (r = 0.98) with those by the manual kit method. The reference interval for 150 apparently normal individuals was 0.12-0.91 U/L. Total CVs of the method ranged from 4.0% to 13.1%. Bilirubin and glucose decreased the CPA activity in serum by as much as 98% and 26%, respectively. Substantial CPA activity was found in pancreatic tissue, with little activity in intestinal tissue. CPA activity was not as widely distributed in extra-pancreatic tissues as were amylase and lipase activities. Peak activities of CPA, amylase, and lipase in the sera of patients with acute pancreatitis were significantly correlated (r = 0.45 to 0.78, P

Original languageEnglish (US)
Pages (from-to)251-255
Number of pages5
JournalClinical Chemistry
Volume35
Issue number2
StatePublished - 1989
Externally publishedYes

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Carboxypeptidases A
Amylases
Tissue
Lipase
Serum
Bilirubin
Pancreatitis
Glucose

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Measuring carboxypeptidase A activity with a centrifugal analyzer : Analytical and clinical considerations. / Kazmierczak, Steven (Steve); Van Lente, F.

In: Clinical Chemistry, Vol. 35, No. 2, 1989, p. 251-255.

Research output: Contribution to journalArticle

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