Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy

J. C. Livesey, R. N. Golden, E. G. Shankland, Z. Grunbaum, T. L. Richards, R. A. Wade, Kenneth Krohn

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethyl-phosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.

Original languageEnglish (US)
Pages (from-to)1937-1940
Number of pages4
JournalCancer Research
Volume49
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

Oxidation-Reduction
Magnetic Resonance Spectroscopy
Sulfhydryl Compounds
Sulfur
Pharmaceutical Preparations
Neoplasms
Carbon
Acids
Liver
Therapeutics
Carbon-13 Magnetic Resonance Spectroscopy
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Livesey, J. C., Golden, R. N., Shankland, E. G., Grunbaum, Z., Richards, T. L., Wade, R. A., & Krohn, K. (1989). Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy. Cancer Research, 49(8), 1937-1940.

Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy. / Livesey, J. C.; Golden, R. N.; Shankland, E. G.; Grunbaum, Z.; Richards, T. L.; Wade, R. A.; Krohn, Kenneth.

In: Cancer Research, Vol. 49, No. 8, 1989, p. 1937-1940.

Research output: Contribution to journalArticle

Livesey, JC, Golden, RN, Shankland, EG, Grunbaum, Z, Richards, TL, Wade, RA & Krohn, K 1989, 'Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy', Cancer Research, vol. 49, no. 8, pp. 1937-1940.
Livesey JC, Golden RN, Shankland EG, Grunbaum Z, Richards TL, Wade RA et al. Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy. Cancer Research. 1989;49(8):1937-1940.
Livesey, J. C. ; Golden, R. N. ; Shankland, E. G. ; Grunbaum, Z. ; Richards, T. L. ; Wade, R. A. ; Krohn, Kenneth. / Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy. In: Cancer Research. 1989 ; Vol. 49, No. 8. pp. 1937-1940.
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