Abstract
The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethylphosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13 C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1937-1940 |
| Number of pages | 4 |
| Journal | Cancer Research |
| Volume | 49 |
| Issue number | 8 |
| State | Published - Sep 1989 |
| Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research