Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy

J. C. Livesey, R. N. Golden, E. G. Shankland, Z. Grunbaum, T. L. Richards, R. A. Wade, Kenneth Krohn

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethyl-phosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.

Original languageEnglish (US)
Pages (from-to)1937-1940
Number of pages4
JournalCancer Research
Volume49
Issue number8
Publication statusPublished - 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Livesey, J. C., Golden, R. N., Shankland, E. G., Grunbaum, Z., Richards, T. L., Wade, R. A., & Krohn, K. (1989). Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy. Cancer Research, 49(8), 1937-1940.