Measurement of blood volume in adult rhesus macaques (macaca mulatta)

Theodore Hobbs, Steven W. Blue, Byung Park, Jennifer J. Greisel, P. Michael Conn, Francis K Y Pau

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Most biomedical facilities that use rhesus macaques (Macaca mulatta) limit the amount of blood that may be collected for experimental purposes. These limits typically are expressed as a percentage of blood volume (BV), estimated by using a fixed ratio of blood (mL) per body weight (kg). BV estimation ratios vary widely among facilities and typically do not factor in variables known to influence BV in humans: sex, age, and body condition. We used indicator dilution methodology to determine the BV of 20 adult rhesus macaques (10 male, 10 female) that varied widely in body condition. We measured body composition by using dual-energy X-ray absorptiometry, weight, crown-to-rump length, and body condition score. Two indicators, FITC-labeled hydroxyethyl starch (FITC-HES) and radioiodinated rhesus serum albumin (125I-RhSA), were injected simultaneously, followed by serial blood collection. Plasma volume at time 0 was determined by linear regression. BV was calculated from the plasma volume and Hct. We found that BV calculated by using FITC-HES was consistently lower than BV calculated by using 125I-RhSA. Sex and age did not significantly affect BV. Percentage body fat was significantly associated with BV. Subjects categorized as having 'optimal' body condition score had 18% body fat and 62.1 mL/kg BV (by FITC-HES; 74.5 mL/kg by 125I-RhSA). Each 1% increase in body fat corresponded to approximately 1 mL/kg decrease in BV. Body condition score correlated with the body fat percentage (R2 = 0.7469). We provide an equation for calculating BV from weight and body condition score.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalJournal of the American Association for Laboratory Animal Science
Volume54
Issue number6
StatePublished - Nov 1 2015

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blood volume
Macaca mulatta
body condition
body fat
starch
blood
dual-energy X-ray absorptiometry
rump
gender
serum albumin
body composition

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Measurement of blood volume in adult rhesus macaques (macaca mulatta). / Hobbs, Theodore; Blue, Steven W.; Park, Byung; Greisel, Jennifer J.; Michael Conn, P.; Pau, Francis K Y.

In: Journal of the American Association for Laboratory Animal Science, Vol. 54, No. 6, 01.11.2015, p. 687-693.

Research output: Contribution to journalArticle

Hobbs, Theodore ; Blue, Steven W. ; Park, Byung ; Greisel, Jennifer J. ; Michael Conn, P. ; Pau, Francis K Y. / Measurement of blood volume in adult rhesus macaques (macaca mulatta). In: Journal of the American Association for Laboratory Animal Science. 2015 ; Vol. 54, No. 6. pp. 687-693.
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