Manipulating and imaging the early Xenopus laevis embryo

Michael V. Danilchik

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Over the past half century, the Xenopus laevis embryo has become a popular model system for studying vertebrate early development at molecular, cellular, and multicellular levels. The year-round availability of easily fertilized eggs, the embryo's large size and rapid development, and the hardiness of both adults and offspring against a wide range of laboratory conditions provide unmatched advantages for a variety of approaches, particularly "cutting and pasting" experiments, to explore embryogenesis. There is, however, a common perception that the Xenopus embryo is intractable for microscope work, due to its store of large, refractile yolk platelets and abundant cortical pigmentation. This chapter presents easily adapted protocols to surmount, and in some cases take advantage of, these optical properties to facilitate live-cell microscopic analysis of commonly used experimental manipulations of early Xenopus embryos.

Original languageEnglish (US)
Title of host publicationVertebrate Embryogenesis
Subtitle of host publicationEmbryological, Cellular, and Genetic Methods
EditorsFrancisco Pelegri
Pages21-54
Number of pages34
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume770
ISSN (Print)1064-3745

Keywords

  • Xenopus laevis
  • cytoskeleton
  • dorsal-ventral axis
  • embryo
  • live-cell confocal imaging
  • time-lapse microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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