Manipulating and imaging the early Xenopus laevis embryo

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Over the past half century, the Xenopus laevis embryo has become a popular model system for studying vertebrate early development at molecular, cellular, and multicellular levels. The year-round availability of easily fertilized eggs, the embryo's large size and rapid development, and the hardiness of both adults and offspring against a wide range of laboratory conditions provide unmatched advantages for a variety of approaches, particularly "cutting and pasting" experiments, to explore embryogenesis. There is, however, a common perception that the Xenopus embryo is intractable for microscope work, due to its store of large, refractile yolk platelets and abundant cortical pigmentation. This chapter presents easily adapted protocols to surmount, and in some cases take advantage of, these optical properties to facilitate live-cell microscopic analysis of commonly used experimental manipulations of early Xenopus embryos.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages21-54
Number of pages34
Volume770
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume770
ISSN (Print)10643745

Fingerprint

Xenopus laevis
Embryonic Structures
Xenopus
Zygote
Pigmentation
Embryonic Development
Vertebrates
Blood Platelets

Keywords

  • cytoskeleton
  • dorsal-ventral axis
  • embryo
  • live-cell confocal imaging
  • time-lapse microscopy
  • Xenopus laevis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Danilchik, M. (2011). Manipulating and imaging the early Xenopus laevis embryo. In Methods in Molecular Biology (Vol. 770, pp. 21-54). (Methods in Molecular Biology; Vol. 770). https://doi.org/10.1007/978-1-61779-210-6_2

Manipulating and imaging the early Xenopus laevis embryo. / Danilchik, Michael.

Methods in Molecular Biology. Vol. 770 2011. p. 21-54 (Methods in Molecular Biology; Vol. 770).

Research output: Chapter in Book/Report/Conference proceedingChapter

Danilchik, M 2011, Manipulating and imaging the early Xenopus laevis embryo. in Methods in Molecular Biology. vol. 770, Methods in Molecular Biology, vol. 770, pp. 21-54. https://doi.org/10.1007/978-1-61779-210-6_2
Danilchik M. Manipulating and imaging the early Xenopus laevis embryo. In Methods in Molecular Biology. Vol. 770. 2011. p. 21-54. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-210-6_2
Danilchik, Michael. / Manipulating and imaging the early Xenopus laevis embryo. Methods in Molecular Biology. Vol. 770 2011. pp. 21-54 (Methods in Molecular Biology).
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