Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules

Young Yang, Klaus Frueh, James Chambers, James B. Waters, Lin Wu, Thomas Spies, Per A. Peterson

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

The mutant murine lymphoma cell line RMA-S is unable to present endogenous antigens due to its inability to efficiently assemble class I major histocompatibility complex molecules and antigenic peptides. Therefore, it has been suggested that RMA-S cells are defective either in peptide generation or in peptide transport into the endoplasmic reticulum, where class I major histocompatibility complex molecule assembly is believed to occur. As proteasomes and the putative peptide transporters HAM1 and HAM2 have been implicated in class I antigen processing, we have investigated their expression in RMA-S and its wild-type counterpart RMA. Both proteasomes and HAM1 proteins are expressed at similar levels and show identical subcellular distributions in the two cell lines. However, only one copy of the HAM2 gene is present in RMA-S cells, and it contains a point mutation that leads to a premature stop codon. Thus, the HAM2 protein is absent from RMA-S cells. These data demonstrate that HAM2 is essential for peptide loading onto class I molecules.

Original languageEnglish (US)
Pages (from-to)11669-11672
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number17
StatePublished - Jun 15 1992
Externally publishedYes

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Major Histocompatibility Complex
Peptides
Molecules
Proteasome Endopeptidase Complex
Cells
Cell Line
Histocompatibility Antigens Class I
Nonsense Codon
Antigen Presentation
Point Mutation
Endoplasmic Reticulum
Lymphoma
Proteins
Genes
Antigens
Processing

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yang, Y., Frueh, K., Chambers, J., Waters, J. B., Wu, L., Spies, T., & Peterson, P. A. (1992). Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules. Journal of Biological Chemistry, 267(17), 11669-11672.

Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules. / Yang, Young; Frueh, Klaus; Chambers, James; Waters, James B.; Wu, Lin; Spies, Thomas; Peterson, Per A.

In: Journal of Biological Chemistry, Vol. 267, No. 17, 15.06.1992, p. 11669-11672.

Research output: Contribution to journalArticle

Yang, Y, Frueh, K, Chambers, J, Waters, JB, Wu, L, Spies, T & Peterson, PA 1992, 'Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules', Journal of Biological Chemistry, vol. 267, no. 17, pp. 11669-11672.
Yang, Young ; Frueh, Klaus ; Chambers, James ; Waters, James B. ; Wu, Lin ; Spies, Thomas ; Peterson, Per A. / Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 17. pp. 11669-11672.
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