Macrophage deficiency of Akt2 reduces atherosclerosis in Ldlr null mice

Vladimir R. Babaev, Katie E. Hebron, Carrie B. Wiese, Cynthia L. Toth, Lei Ding, Youmin Zhang, James M. May, Sergio Fazio, Kasey C. Vickers, MacRae F. Linton

    Research output: Contribution to journalArticle

    34 Scopus citations

    Abstract

    Macrophages play crucial roles in the formation of atherosclerotic lesions. Akt, a serine/threonine protein kinase B, is vital for cell proliferation, migration, and survival. Macrophages express three Akt isoforms, Akt1, Akt2, and Akt3, but the roles of Akt1 and Akt2 in atherosclerosis in vivo remain unclear. To dissect the impact of macrophage Akt1 and Akt2 on early atherosclerosis, we generated mice with hematopoietic deficiency of Akt1 or Akt2. After 8 weeks on Western diet, Ldlr-/- mice reconstituted with Akt1-/- fetal liver cells ( Akt1-/-→Ldlr-/-) had similar atherosclerotic lesion areas compared with control mice transplanted with WT cells (WT→Ldlr-/-). In contrast, Akt2-/- →Ldlr-/- mice had dramatically reduced atherosclerotic lesions compared with WT→Ldlr-/- mice of both genders. Similarly, in the setting of advanced atherosclerotic lesions, Akt2-/-→Ldlr-/- mice had smaller aortic lesions compared with WT→Ldlr-/- and Akt1-/-→Ldlr-/- mice. Importantly, Akt2-/-→Ldlr-/- mice had reduced numbers of proinflammatory blood monocytes expressing Ly-6Chi and chemokine C-C motif receptor 2. Peritoneal macrophages isolated from Akt2-/- mice were skewed toward an M2 phenotype and showed decreased expression of proinflammatory genes and reduced cell migration. Our data demonstrate that loss of Akt2 suppresses the ability of macrophages to undergo M1 polarization reducing both early and advanced atherosclerosis.

    Original languageEnglish (US)
    Pages (from-to)2296-2308
    Number of pages13
    JournalJournal of lipid research
    Volume55
    Issue number11
    DOIs
    StatePublished - 2014

    Keywords

    • Apoptosis
    • Cell signaling
    • Foam cells
    • Macrophages/monocytes

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Cell Biology

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  • Cite this

    Babaev, V. R., Hebron, K. E., Wiese, C. B., Toth, C. L., Ding, L., Zhang, Y., May, J. M., Fazio, S., Vickers, K. C., & Linton, M. F. (2014). Macrophage deficiency of Akt2 reduces atherosclerosis in Ldlr null mice. Journal of lipid research, 55(11), 2296-2308. https://doi.org/10.1194/jlr.M050633