Loss of HLA class‐I alleles, heavy chains and β2‐microglobulin in colorectal cancer

Loukas Kaklamanis, Kevin C. Gatter, Ann B. Hill, Neil Mortensen, Adrian L. Harris, Peter Krausa, Andrew McMichael, Julia G. Bodmer, Walter F. Bodmer

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55 Scopus citations

Abstract

Using immunohistochemical methods, we have analysed colorectal biopsies of normal mucosa, metaplastic polyps (5 cases), adenomas (15 cases) and adenocarcinomas (70 cases) with 13 monoclonal antibodies (MAbs) to allelic products of the HLA‐A, B, C loci. Nine of the 70 carcinomas showed total loss of HLA Class‐I molecules due to an underlying defect regarding not only the expression of β2‐microglobulin (·β2‐m), but also the heavy chains of HLA A, B and C loci, or both. Much commoner was a loss of one or more Class‐I alleles as follows: Al/Aw36 (completely lost in 4 of 29 cases and focally lost in another 2), A2 (in 1 of 37 cases), A3 (in 2 of 14 cases), A11/28/31/33 (in 3 of 11 cases), B7 (in 3 of 13 and focally in 1 other case), B17 (in 1 case), Bw4 (in 8 of 45 and focally in another 6), Bw6 (in 9 of 62 and focally in another 3). Focal selective loss (Bw6 and a combined A1‐Bw6), was observed in 2 adenomas. Normal colonic mucosa, as well as stromal and lymphoid cells present between the neoplastic glands, were studied in each case as a control. A particular allele was only considered to be lost by the malignant cells if it was still expressed on these adjacent tissues.

Original languageEnglish (US)
Pages (from-to)379-385
Number of pages7
JournalInternational Journal of Cancer
Volume51
Issue number3
DOIs
StatePublished - May 28 1992

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kaklamanis, L., Gatter, K. C., Hill, A. B., Mortensen, N., Harris, A. L., Krausa, P., McMichael, A., Bodmer, J. G., & Bodmer, W. F. (1992). Loss of HLA class‐I alleles, heavy chains and β2‐microglobulin in colorectal cancer. International Journal of Cancer, 51(3), 379-385. https://doi.org/10.1002/ijc.2910510308