Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis

Rajesha Rupaimoole, Jaehyuk Lee, Monika Haemmerle, Hui Ling, Rebecca A. Previs, Sunila Pradeep, Sherry Y. Wu, Cristina Ivan, Manuela Ferracin, Jennifer B. Dennison, Niki M.Zacharias Millward, Archana S. Nagaraja, Kshipra M. Gharpure, Michael McGuire, Nidhin Sam, Guillermo N. Armaiz-Pena, Nouara C. Sadaoui, Cristian Rodriguez-Aguayo, George A. Calin, Ronny I. Drapkin & 6 others Jeffery Kovacs, Gordon Mills, Wei Zhang, Gabriel Lopez-Berestein, Pratip K. Bhattacharya, Anil K. Sood

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Long noncoding RNAs (lncRNAs) significantly influence the development and regulation of genome expression in cells. Here, we demonstrate the role of lncRNA ceruloplasmin (NRCP) in cancer metabolism and elucidate functional effects leading to increased tumor progression. NRCP was highly upregulated in ovarian tumors, and knockdown of NRCP resulted in significantly increased apoptosis, decreased cell proliferation, and decreased glycolysis compared with control cancer cells. In an orthotopic mouse model of ovarian cancer, siNRCP delivered via a liposomal carrier significantly reduced tumor growth compared with control treatment. We identified NRCP as an intermediate binding partner between STAT1 and RNA polymerase II, leading to increased expression of downstream target genes such as glucose-6-phosphate isomerase. Collectively, we report a previously unrecognized role of the lncRNA NRCP in modulating cancer metabolism. As demonstrated, DOPC nanoparticle-incorporated siRNA-mediated silencing of this lncRNA in vivo provides therapeutic avenue toward modulating lncRNAs in cancer.

Original languageEnglish (US)
Pages (from-to)2395-2402
Number of pages8
JournalCell Reports
Volume13
Issue number11
DOIs
StatePublished - Dec 22 2015
Externally publishedYes

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Long Noncoding RNA
Ceruloplasmin
Glycolysis
Tumors
Growth
Neoplasms
Metabolism
Genes
Glucose-6-Phosphate Isomerase
RNA Polymerase II
Cell proliferation
Small Interfering RNA
Cells
Apoptosis
Nanoparticles
Ovarian Neoplasms
Cell Proliferation
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rupaimoole, R., Lee, J., Haemmerle, M., Ling, H., Previs, R. A., Pradeep, S., ... Sood, A. K. (2015). Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis. Cell Reports, 13(11), 2395-2402. https://doi.org/10.1016/j.celrep.2015.11.047

Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis. / Rupaimoole, Rajesha; Lee, Jaehyuk; Haemmerle, Monika; Ling, Hui; Previs, Rebecca A.; Pradeep, Sunila; Wu, Sherry Y.; Ivan, Cristina; Ferracin, Manuela; Dennison, Jennifer B.; Millward, Niki M.Zacharias; Nagaraja, Archana S.; Gharpure, Kshipra M.; McGuire, Michael; Sam, Nidhin; Armaiz-Pena, Guillermo N.; Sadaoui, Nouara C.; Rodriguez-Aguayo, Cristian; Calin, George A.; Drapkin, Ronny I.; Kovacs, Jeffery; Mills, Gordon; Zhang, Wei; Lopez-Berestein, Gabriel; Bhattacharya, Pratip K.; Sood, Anil K.

In: Cell Reports, Vol. 13, No. 11, 22.12.2015, p. 2395-2402.

Research output: Contribution to journalArticle

Rupaimoole, R, Lee, J, Haemmerle, M, Ling, H, Previs, RA, Pradeep, S, Wu, SY, Ivan, C, Ferracin, M, Dennison, JB, Millward, NMZ, Nagaraja, AS, Gharpure, KM, McGuire, M, Sam, N, Armaiz-Pena, GN, Sadaoui, NC, Rodriguez-Aguayo, C, Calin, GA, Drapkin, RI, Kovacs, J, Mills, G, Zhang, W, Lopez-Berestein, G, Bhattacharya, PK & Sood, AK 2015, 'Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis', Cell Reports, vol. 13, no. 11, pp. 2395-2402. https://doi.org/10.1016/j.celrep.2015.11.047
Rupaimoole R, Lee J, Haemmerle M, Ling H, Previs RA, Pradeep S et al. Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis. Cell Reports. 2015 Dec 22;13(11):2395-2402. https://doi.org/10.1016/j.celrep.2015.11.047
Rupaimoole, Rajesha ; Lee, Jaehyuk ; Haemmerle, Monika ; Ling, Hui ; Previs, Rebecca A. ; Pradeep, Sunila ; Wu, Sherry Y. ; Ivan, Cristina ; Ferracin, Manuela ; Dennison, Jennifer B. ; Millward, Niki M.Zacharias ; Nagaraja, Archana S. ; Gharpure, Kshipra M. ; McGuire, Michael ; Sam, Nidhin ; Armaiz-Pena, Guillermo N. ; Sadaoui, Nouara C. ; Rodriguez-Aguayo, Cristian ; Calin, George A. ; Drapkin, Ronny I. ; Kovacs, Jeffery ; Mills, Gordon ; Zhang, Wei ; Lopez-Berestein, Gabriel ; Bhattacharya, Pratip K. ; Sood, Anil K. / Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis. In: Cell Reports. 2015 ; Vol. 13, No. 11. pp. 2395-2402.
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