Long-Lived Plasma Cells Are Contained within the CD19-CD38hiCD138+ Subset in Human Bone Marrow

Jessica L. Halliley, Christopher M. Tipton, Jane Liesveld, Alexander F. Rosenberg, Jaime Darce, Ivan V. Gregoretti, Lana Popova, Denise Kaminiski, Christopher F. Fucile, Igor Albizua, Shuya Kyu, Kuang Yueh Chiang, Kyle T. Bradley, Richard Burack, Mark Slifka, Erika Hammarlund, Hao Wu, Liping Zhao, Edward E. Walsh, Ann R. FalseyTroy D. Randall, Wan Cheung Cheung, Iñaki Sanz, F. Eun Hyung Lee

    Research output: Contribution to journalArticle

    134 Scopus citations

    Abstract

    Antibody responses to viral infections are sustained for decades by long-lived plasma cells (LLPCs). However, LLPCs have yet to be characterized in humans. Here we used CD19, CD38, and CD138 to identify four PC subsets in humanbone marrow (BM). We found that the CD19-CD38hiCD138+ subset was morphologically distinct, differentially expressed PC-associated genes, and exclusively contained PCs specific for viral antigens to which the subjects had not beenexposed for more than 40 years. Protein sequences of measles- and mumps-specific circulating antibodies were encoded for by CD19-CD38hiCD138+ PCs in the BM. Finally, we found that CD19-CD38hiCD138+ PCs had a distinct RNA transcriptome signature and human immunoglobulin heavy chain (VH) repertoire that was relatively uncoupled from other BM PC subsets and probably represents the B cell response's "historical record" of antigenic exposure. Thus, our studies define human LLPCs and provide a mechanism for the life-long maintenance of anti-viral antibodies in the serum.

    Original languageEnglish (US)
    Pages (from-to)132-145
    Number of pages14
    JournalImmunity
    Volume43
    Issue number1
    DOIs
    StatePublished - Jul 21 2015

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    Keywords

    • Antibody secreting cells
    • Bone marrow
    • Heterogeneity
    • Human
    • Long-lived plasma cells
    • Measles
    • Mumps infection
    • Next generation sequencing
    • Plasma cells
    • Plasmablasts
    • Proteomics
    • Vaccine

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Infectious Diseases

    Cite this

    Halliley, J. L., Tipton, C. M., Liesveld, J., Rosenberg, A. F., Darce, J., Gregoretti, I. V., Popova, L., Kaminiski, D., Fucile, C. F., Albizua, I., Kyu, S., Chiang, K. Y., Bradley, K. T., Burack, R., Slifka, M., Hammarlund, E., Wu, H., Zhao, L., Walsh, E. E., ... Lee, F. E. H. (2015). Long-Lived Plasma Cells Are Contained within the CD19-CD38hiCD138+ Subset in Human Bone Marrow. Immunity, 43(1), 132-145. https://doi.org/10.1016/j.immuni.2015.06.016