Localization of the Plasmodium falciparum PfNT1 Nucleoside Transporter to the Parasite Plasma Membrane

Nicolle Rager, Choukri Ben Mamoun, Nicola Carter, Daniel E. Goldberg, Buddy Ullman

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Nutrient transporters play critical roles in parasite metabolism, but the membranes in which they reside have not been clearly defined. The transport of purine nutrients is crucial to the survival of the malaria parasite Plasmodium falciparum, and nucleoside transport activity has been associated with a number of different membrane components within the parasitized erythrocyte. To determine the location of the PfNT1 nucleoside transporter, the first component of the nucleoside permeation pathway to be studied at the molecular level in P. falciparum (Carter, N. S., Ben Mamoun, C., Liu, W., Silva, E. O., Landfear, S. M., Goldberg, D. E., and Ullman, B. (2000) J. Biol. Chem. 275, 10683-10691), polyclonal antisera against the NH2-terminal 36 amino acids of PfNT1 were raised in rabbits. Western blot analysis of parasite lysates revealed that the antibodies were specific for PfNT1 and that the level of PfNT1 protein in the infected erythrocyte is regulated in a stage-specific fashion. The amount of PfNT1 polypeptide increases dramatically during the early trophozoite stage and reaches its maximal level in the late trophozoite and schizont stages. Deconvolution and immunoelectron microscopy using these monospecific antibodies revealed that PfNT1 localizes predominantly, if not exclusively, to the plasma membrane of the parasite and not to the parasitophorous vacuolar or erythrocyte membranes.

Original languageEnglish (US)
Pages (from-to)41095-41099
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number44
DOIs
StatePublished - Nov 2 2001

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Nucleoside Transport Proteins
Cell membranes
Plasmodium falciparum
Parasites
Cell Membrane
Trophozoites
Membranes
Nucleosides
Nutrients
Erythrocytes
Schizonts
Food
Immunoelectron Microscopy
Antibodies
Falciparum Malaria
Erythrocyte Membrane
Deconvolution
Metabolism
Permeation
Immune Sera

ASJC Scopus subject areas

  • Biochemistry

Cite this

Localization of the Plasmodium falciparum PfNT1 Nucleoside Transporter to the Parasite Plasma Membrane. / Rager, Nicolle; Ben Mamoun, Choukri; Carter, Nicola; Goldberg, Daniel E.; Ullman, Buddy.

In: Journal of Biological Chemistry, Vol. 276, No. 44, 02.11.2001, p. 41095-41099.

Research output: Contribution to journalArticle

Rager, Nicolle ; Ben Mamoun, Choukri ; Carter, Nicola ; Goldberg, Daniel E. ; Ullman, Buddy. / Localization of the Plasmodium falciparum PfNT1 Nucleoside Transporter to the Parasite Plasma Membrane. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 44. pp. 41095-41099.
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