Localization of fibrillin-I in the human term placenta

Sig Linda Jacobson, Dwight Kimberly, Kent Thornburg, Cheryl Maslen

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Objective: We determined the level of message for fibrillin-1, a single-stranded glycoprotein found in the elastic microfibrils of the extracellular matrix, in human placenta compared with other adult human tissues and localized the protein in the term human placenta. Methods: Northern blot analysis using a cDNA specific for fibrillin-1 was used to compare message levels in several adult human tissues and placenta. Immunohistochemical staining, using a monoclonal antibody to human fibrillin-1, was used to visualize the site of fibrillin-1 in term human placenta. Results: Larger amounts of message for fibrillin-1 were present in placenta than in any of the other tissues tested. Staining for fibrillin-1 was present in the villous stroma but not in the basement membranes, trophoblast cells, or in blood vessels of stem, mature intermediate, or terminal villi. Conclusions: Fibrillin-1 is distributed extensively in the villous stroma of the term human placenta and may contribute elastic properties to the placenta and basal plate required for an active uterus.

    Original languageEnglish (US)
    Pages (from-to)686-690
    Number of pages5
    JournalJournal of the Society for Gynecologic Investigation
    Volume2
    Issue number5
    StatePublished - Sep 1995

    Fingerprint

    Placenta
    Staining and Labeling
    Microfibrils
    Trophoblasts
    Fibrillins
    Fibrillin-1
    Basement Membrane
    Northern Blotting
    Uterus
    Extracellular Matrix
    Blood Vessels
    Glycoproteins
    Complementary DNA
    Monoclonal Antibodies
    Proteins

    Keywords

    • extracellular matrix
    • fibrillin-1
    • immunohistochemistry
    • microfibril
    • Placenta

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology
    • Developmental Biology

    Cite this

    Localization of fibrillin-I in the human term placenta. / Jacobson, Sig Linda; Kimberly, Dwight; Thornburg, Kent; Maslen, Cheryl.

    In: Journal of the Society for Gynecologic Investigation, Vol. 2, No. 5, 09.1995, p. 686-690.

    Research output: Contribution to journalArticle

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    abstract = "Objective: We determined the level of message for fibrillin-1, a single-stranded glycoprotein found in the elastic microfibrils of the extracellular matrix, in human placenta compared with other adult human tissues and localized the protein in the term human placenta. Methods: Northern blot analysis using a cDNA specific for fibrillin-1 was used to compare message levels in several adult human tissues and placenta. Immunohistochemical staining, using a monoclonal antibody to human fibrillin-1, was used to visualize the site of fibrillin-1 in term human placenta. Results: Larger amounts of message for fibrillin-1 were present in placenta than in any of the other tissues tested. Staining for fibrillin-1 was present in the villous stroma but not in the basement membranes, trophoblast cells, or in blood vessels of stem, mature intermediate, or terminal villi. Conclusions: Fibrillin-1 is distributed extensively in the villous stroma of the term human placenta and may contribute elastic properties to the placenta and basal plate required for an active uterus.",
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    AU - Kimberly, Dwight

    AU - Thornburg, Kent

    AU - Maslen, Cheryl

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    N2 - Objective: We determined the level of message for fibrillin-1, a single-stranded glycoprotein found in the elastic microfibrils of the extracellular matrix, in human placenta compared with other adult human tissues and localized the protein in the term human placenta. Methods: Northern blot analysis using a cDNA specific for fibrillin-1 was used to compare message levels in several adult human tissues and placenta. Immunohistochemical staining, using a monoclonal antibody to human fibrillin-1, was used to visualize the site of fibrillin-1 in term human placenta. Results: Larger amounts of message for fibrillin-1 were present in placenta than in any of the other tissues tested. Staining for fibrillin-1 was present in the villous stroma but not in the basement membranes, trophoblast cells, or in blood vessels of stem, mature intermediate, or terminal villi. Conclusions: Fibrillin-1 is distributed extensively in the villous stroma of the term human placenta and may contribute elastic properties to the placenta and basal plate required for an active uterus.

    AB - Objective: We determined the level of message for fibrillin-1, a single-stranded glycoprotein found in the elastic microfibrils of the extracellular matrix, in human placenta compared with other adult human tissues and localized the protein in the term human placenta. Methods: Northern blot analysis using a cDNA specific for fibrillin-1 was used to compare message levels in several adult human tissues and placenta. Immunohistochemical staining, using a monoclonal antibody to human fibrillin-1, was used to visualize the site of fibrillin-1 in term human placenta. Results: Larger amounts of message for fibrillin-1 were present in placenta than in any of the other tissues tested. Staining for fibrillin-1 was present in the villous stroma but not in the basement membranes, trophoblast cells, or in blood vessels of stem, mature intermediate, or terminal villi. Conclusions: Fibrillin-1 is distributed extensively in the villous stroma of the term human placenta and may contribute elastic properties to the placenta and basal plate required for an active uterus.

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