Papel local dos androgênios no desenvolvimento de folículos pré-antrais de primatas não humanos cultivados individualmente em matriz tridimensional

Translated title of the contribution: Local role of androgens in the development of preantral follicles from nonhuman primates cultured individually a in three-dimensional matrix

Jhenifer Kliemchen Rodrigues, Paula Andrea De Albuquerque Salles Navarro, Mary Zelinski, Richard Stouffer, Jing Xu

    Research output: Contribution to journalArticle

    Abstract

    Objectives To evaluate the effects of ablation of steroid production and the role of testosterone and dihydrotestosterone in the development of secondary follicles of nonhuman primates (Macaca mulatta) in 3 D matrix. Methods The ovarian cortex (n = 8 adult females) was diced and secondary follicles were isolated and cultured individually for 40 days on alginate matrix. Experiment 1: Ablation of steroid production with use of trilostane (TRL) and testosterone replacement. Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), Low testosterone (T) (TRL testosterone and 10 ng/mL), high T (TRL testosterone and 50 ng/mL); Experiment 2: Ablation of steroid production with use of trilostane (TRL) and replacement of dihydrotestosterone (DHT). Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), DHT (DHT 50 ng/mL), DHT + TRL (TRL and DHT 50 ng/mL). It were analyzed: survival, growth, antrum formation, steroid hormone production, and degree of maturation and quality of oocytes. Results The percentage of surviving follicles in culture, growth and antrum formation was lower in the presence of TRL in all experiments, compared with the control group. Replacement of T and DHT in the presence of TRL, recovered survival, growth, antrum formation, hormone production, and the quality and maturity of oocytes comparable to the control group. DHT added to standard maturation media promoted an increase in the percentage of survival follicles and antrum formation compared to the control group. Conclusions This study provided evidence of the role of androgens since the beginning of folliculogenesis in primates, reinforcing the existence of molecular mechanisms regulating androgenic activity, and new interactions between androgens and steroid hormones. Our findings show that T and DHT can recover survival, growth, antrum formation, hormone production and oocyte viability of in vitro cultured preantral follicles exposed to ablation of steroid production. DHT added to standard maturation media promoted an increase in the percentage of follicle survival and antrum formation compared to the control group, opening perspectives to evaluate whether this androgen supplementation could improve follicular development in vitro. The knowledge obtained contribute to a understanding of the dynamics of folliculogenesis in primates, still poorly understood, and assists in identifying the optimal conditions for growing follicle, which may in future be applied to assist cancer patients who wish to preserve their future fertility.

    Original languagePortuguese
    Pages (from-to)93-104
    Number of pages12
    JournalReproducao e Climaterio
    Volume29
    Issue number3
    DOIs
    StatePublished - 2014

    Fingerprint

    Dihydrotestosterone
    Primates
    Androgens
    Steroids
    Testosterone
    Control Groups
    Survival
    Hormones
    Oocytes
    Growth
    trilostane
    Macaca mulatta
    Fertility

    Keywords

    • 3D follicle culture
    • Androgens
    • Dihydrotestosterone
    • Folliculogenesis
    • Primates
    • Steroids
    • Testosterone

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Endocrinology, Diabetes and Metabolism
    • Reproductive Medicine

    Cite this

    Papel local dos androgênios no desenvolvimento de folículos pré-antrais de primatas não humanos cultivados individualmente em matriz tridimensional. / Rodrigues, Jhenifer Kliemchen; Navarro, Paula Andrea De Albuquerque Salles; Zelinski, Mary; Stouffer, Richard; Xu, Jing.

    In: Reproducao e Climaterio, Vol. 29, No. 3, 2014, p. 93-104.

    Research output: Contribution to journalArticle

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    title = "Papel local dos androg{\^e}nios no desenvolvimento de fol{\'i}culos pr{\'e}-antrais de primatas n{\~a}o humanos cultivados individualmente em matriz tridimensional",
    abstract = "Objectives To evaluate the effects of ablation of steroid production and the role of testosterone and dihydrotestosterone in the development of secondary follicles of nonhuman primates (Macaca mulatta) in 3 D matrix. Methods The ovarian cortex (n = 8 adult females) was diced and secondary follicles were isolated and cultured individually for 40 days on alginate matrix. Experiment 1: Ablation of steroid production with use of trilostane (TRL) and testosterone replacement. Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), Low testosterone (T) (TRL testosterone and 10 ng/mL), high T (TRL testosterone and 50 ng/mL); Experiment 2: Ablation of steroid production with use of trilostane (TRL) and replacement of dihydrotestosterone (DHT). Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), DHT (DHT 50 ng/mL), DHT + TRL (TRL and DHT 50 ng/mL). It were analyzed: survival, growth, antrum formation, steroid hormone production, and degree of maturation and quality of oocytes. Results The percentage of surviving follicles in culture, growth and antrum formation was lower in the presence of TRL in all experiments, compared with the control group. Replacement of T and DHT in the presence of TRL, recovered survival, growth, antrum formation, hormone production, and the quality and maturity of oocytes comparable to the control group. DHT added to standard maturation media promoted an increase in the percentage of survival follicles and antrum formation compared to the control group. Conclusions This study provided evidence of the role of androgens since the beginning of folliculogenesis in primates, reinforcing the existence of molecular mechanisms regulating androgenic activity, and new interactions between androgens and steroid hormones. Our findings show that T and DHT can recover survival, growth, antrum formation, hormone production and oocyte viability of in vitro cultured preantral follicles exposed to ablation of steroid production. DHT added to standard maturation media promoted an increase in the percentage of follicle survival and antrum formation compared to the control group, opening perspectives to evaluate whether this androgen supplementation could improve follicular development in vitro. The knowledge obtained contribute to a understanding of the dynamics of folliculogenesis in primates, still poorly understood, and assists in identifying the optimal conditions for growing follicle, which may in future be applied to assist cancer patients who wish to preserve their future fertility.",
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    AU - Rodrigues, Jhenifer Kliemchen

    AU - Navarro, Paula Andrea De Albuquerque Salles

    AU - Zelinski, Mary

    AU - Stouffer, Richard

    AU - Xu, Jing

    PY - 2014

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    N2 - Objectives To evaluate the effects of ablation of steroid production and the role of testosterone and dihydrotestosterone in the development of secondary follicles of nonhuman primates (Macaca mulatta) in 3 D matrix. Methods The ovarian cortex (n = 8 adult females) was diced and secondary follicles were isolated and cultured individually for 40 days on alginate matrix. Experiment 1: Ablation of steroid production with use of trilostane (TRL) and testosterone replacement. Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), Low testosterone (T) (TRL testosterone and 10 ng/mL), high T (TRL testosterone and 50 ng/mL); Experiment 2: Ablation of steroid production with use of trilostane (TRL) and replacement of dihydrotestosterone (DHT). Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), DHT (DHT 50 ng/mL), DHT + TRL (TRL and DHT 50 ng/mL). It were analyzed: survival, growth, antrum formation, steroid hormone production, and degree of maturation and quality of oocytes. Results The percentage of surviving follicles in culture, growth and antrum formation was lower in the presence of TRL in all experiments, compared with the control group. Replacement of T and DHT in the presence of TRL, recovered survival, growth, antrum formation, hormone production, and the quality and maturity of oocytes comparable to the control group. DHT added to standard maturation media promoted an increase in the percentage of survival follicles and antrum formation compared to the control group. Conclusions This study provided evidence of the role of androgens since the beginning of folliculogenesis in primates, reinforcing the existence of molecular mechanisms regulating androgenic activity, and new interactions between androgens and steroid hormones. Our findings show that T and DHT can recover survival, growth, antrum formation, hormone production and oocyte viability of in vitro cultured preantral follicles exposed to ablation of steroid production. DHT added to standard maturation media promoted an increase in the percentage of follicle survival and antrum formation compared to the control group, opening perspectives to evaluate whether this androgen supplementation could improve follicular development in vitro. The knowledge obtained contribute to a understanding of the dynamics of folliculogenesis in primates, still poorly understood, and assists in identifying the optimal conditions for growing follicle, which may in future be applied to assist cancer patients who wish to preserve their future fertility.

    AB - Objectives To evaluate the effects of ablation of steroid production and the role of testosterone and dihydrotestosterone in the development of secondary follicles of nonhuman primates (Macaca mulatta) in 3 D matrix. Methods The ovarian cortex (n = 8 adult females) was diced and secondary follicles were isolated and cultured individually for 40 days on alginate matrix. Experiment 1: Ablation of steroid production with use of trilostane (TRL) and testosterone replacement. Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), Low testosterone (T) (TRL testosterone and 10 ng/mL), high T (TRL testosterone and 50 ng/mL); Experiment 2: Ablation of steroid production with use of trilostane (TRL) and replacement of dihydrotestosterone (DHT). Follicles of 4 animals were assigned to 4 groups: control (vehicle), TRL (TRL from the start of culture), DHT (DHT 50 ng/mL), DHT + TRL (TRL and DHT 50 ng/mL). It were analyzed: survival, growth, antrum formation, steroid hormone production, and degree of maturation and quality of oocytes. Results The percentage of surviving follicles in culture, growth and antrum formation was lower in the presence of TRL in all experiments, compared with the control group. Replacement of T and DHT in the presence of TRL, recovered survival, growth, antrum formation, hormone production, and the quality and maturity of oocytes comparable to the control group. DHT added to standard maturation media promoted an increase in the percentage of survival follicles and antrum formation compared to the control group. Conclusions This study provided evidence of the role of androgens since the beginning of folliculogenesis in primates, reinforcing the existence of molecular mechanisms regulating androgenic activity, and new interactions between androgens and steroid hormones. Our findings show that T and DHT can recover survival, growth, antrum formation, hormone production and oocyte viability of in vitro cultured preantral follicles exposed to ablation of steroid production. DHT added to standard maturation media promoted an increase in the percentage of follicle survival and antrum formation compared to the control group, opening perspectives to evaluate whether this androgen supplementation could improve follicular development in vitro. The knowledge obtained contribute to a understanding of the dynamics of folliculogenesis in primates, still poorly understood, and assists in identifying the optimal conditions for growing follicle, which may in future be applied to assist cancer patients who wish to preserve their future fertility.

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    KW - Androgens

    KW - Dihydrotestosterone

    KW - Folliculogenesis

    KW - Primates

    KW - Steroids

    KW - Testosterone

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