Local presentation of substrate molecules directs axon specification by cultured hippocampal neurons

Teresa Esch, Vance Lemmon, Gary Banker

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Axon specification is a crucial, early step in neuronal development, but little is known about how this event is controlled in vivo. To test the hypothesis that local presentation of growth-promoting molecules can direct axon specification, we cultured hippocampal neurons on substrates patterned with stripes of poly-L-lysine and either laminin (LN) or the neuron-glia cell adhesion molecule (NgCAM). Although undifferentiated neurites contacted both substrates equally, axons formed preferentially on LN or NgCAM. Time-lapse studies revealed that changes in the growth pattern of a cell indicative of axon specification began almost immediately after the growth cone of one of the neurites of the cell contacted LN or NgCAM. When cells were plated on alternating stripes of LN and NgCAM, cells with their somata on LN usually formed axons on NgCAM, whereas those with somata on NgCAM preferentially formed axons on LN. This suggests that the change from one axon-promoting substrate to another also provides a signal sufficient to specify the axon. These results demonstrate that contact with preferred substrate molecules can govern which neurite becomes the axon and thus direct the development of neuronal polarity.

Original languageEnglish (US)
Pages (from-to)6417-6426
Number of pages10
JournalJournal of Neuroscience
Volume19
Issue number15
StatePublished - Aug 1 1999

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Neuron-Glia Cell Adhesion Molecules
Axons
Laminin
Neurons
Neurites
Carisoprodol
Body Patterning
Growth Cones
Growth
Lysine

Keywords

  • Axonal specification
  • Hippocampal cultures
  • Laminin
  • Neuronal development
  • NgCAM
  • Polarity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Local presentation of substrate molecules directs axon specification by cultured hippocampal neurons. / Esch, Teresa; Lemmon, Vance; Banker, Gary.

In: Journal of Neuroscience, Vol. 19, No. 15, 01.08.1999, p. 6417-6426.

Research output: Contribution to journalArticle

Esch, Teresa ; Lemmon, Vance ; Banker, Gary. / Local presentation of substrate molecules directs axon specification by cultured hippocampal neurons. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 15. pp. 6417-6426.
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