Local implants of testosterone metabolites regulate vasopressin mRNA in sexually dimorphic nuclei of the rat brain

M. D. Brot, G. J. De Vries, Daniel Dorsa

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Biosynthesis of the neuropeptide vasopressin (VP) in extrahypothalamic neurons is dependent on circulating levels of testosterone (T). However, the mechanism by which endogenous or peripherally administered T induces VP gene expression in the brain has not been established. This study investigated the effects of androgens and estrogen in the steroid-dependent expression of VP mRNA in the bed nucleus of the stria terminalis (BNST). Testosterone, estrogen, and the T metabolite, dihydrotestosterone (DHT), were either peripherally administered or locally implanted in cannula into the BNST of castrated male rats to determine whether these steroids influence VP gene expression through a local effect within the nucleus itself. The results indicate that T does act locally within the BNST, since complete restoration of VP mRNA levels occurred in BNST neurons in the vicinity of T-containing cannulas but not on the contralateral side. In addition, both DHT and estrogen were partially effective in stimulating VP gene expression in the BNST, and in combination, synergized to produce the full complement of VP gene expression induced by T itself.

Original languageEnglish (US)
Pages (from-to)933-940
Number of pages8
JournalPeptides
Volume14
Issue number5
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Metabolites
Vasopressins
Septal Nuclei
Testosterone
Rats
Brain
Messenger RNA
Gene expression
Gene Expression
Estrogens
Dihydrotestosterone
Neurons
Steroids
Biosynthesis
Neuropeptides
Androgens
Restoration

Keywords

  • Androgen
  • Bed nucleus of the stria terminalis (BNST)
  • Cannula
  • Dihydrotestosterone
  • Estrogen
  • Medial amygdada
  • Testosterone
  • Vasopressin mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Local implants of testosterone metabolites regulate vasopressin mRNA in sexually dimorphic nuclei of the rat brain. / Brot, M. D.; De Vries, G. J.; Dorsa, Daniel.

In: Peptides, Vol. 14, No. 5, 1993, p. 933-940.

Research output: Contribution to journalArticle

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