Light and electron microscopy immunolocalization of BFGF in eosinophils in chronic inflammatory tissues

P. Ahmadi, Z. Ou, Michael (Mike) Powers, R. Kayton, L. Mornson

Research output: Contribution to journalArticle

Abstract

Eosinophils are involved in several inflammatory disorders of unknown etiology. However, their exact contribution to inflammation remains largely unknown. Recent studies have shown that eosinophils produce several cytokines/growth factors that are important inflammatory mediators. In the process of identifying cellular sources of basic fibroblast growth factor (bFGF), a potent angiogenic and mitogenic polypeptide, we examined tissue samples from patients with pemphigoid or nasal polyposis, as well as dermal granuloma from rats. In all samples examined, a subpopulation of both ultra- and extra-vascular eosinophils exhibited strong cytoplasmic immunoreactivity to ami-bFGF antibodies. Their identity was confirmed in adjacent 2-(im sequential sections. By electron microscopy, eosinophils were identified by their typical cytoplasmic granules containing a crystalline core. Strong immunostaining for bFGF was found in the granules (excluding the crystalline core), and moderate staining was found in the nucleus. In contrast, no staining was found in lymphocytes or plasma cells in the same tissue. Furthermore, all procedural controls were devoid of staining. These results link bFGF to eosinophils in chronic inflammatory tissues and suggest that they may play a role in regulating tissue bFGF levels. An investigation is currently in progress to determine if bFGF mRNA can also be found in thesft ensinnnhik and if hFGF levels in ensinnnhils and rorrelateri with disease state.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

fibroblast growth factor 2
Fibroblast Growth Factor 2
eosinophils
Eosinophils
Electron microscopy
Optical microscopy
light microscopy
electron microscopy
Electron Microscopy
Tissue
Light
Staining and Labeling
Crystalline materials
cytoplasmic granules
Cytoplasmic Granules
Bullous Pemphigoid
Lymphocytes
plasma cells
granuloma
Plasma Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Light and electron microscopy immunolocalization of BFGF in eosinophils in chronic inflammatory tissues. / Ahmadi, P.; Ou, Z.; Powers, Michael (Mike); Kayton, R.; Mornson, L.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

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